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Team:UTK-Knoxville/1 July 2013
From 2013.igem.org
July 1st, 2013
| Construct Name | Construct Number | Status |
| Aer-EnvZ+OmpR | 1 | Currently being redone, and on plates in Eric's lab |
| HemAT-NtrB+NtrC | 2 | Re-designing primers and extracting gDNA |
| RcoM-NtrB+NtrC | 3 | Re-designing primers and waiting on G. Caspiferriformans gDNA from Eric |
| NarX-EnvZ+OmpR (NarX 1) | 4 | Not started |
| NarX-EnvZ+OmpR (NarX 2) | 5 | Not started |
| TodS-EnvZ+OmpR | 6 | Not started |
| LasR-?+? | 7 | Not started |
Once again, the R Leg strain did not grow. This is unexpected, since I have already seen it grow in the TYC media I made the first time around. I am going to ask Eric about this. The PCR I ran for HemAT at a wider range of temps(55, 60, 65) failed once more. I don’t know what could be causing this issue, so I am going to look into the primers first. After talking to Eric, we have learned that R Leg and Azo are both unable to be stored in extreme cold as they are very sensitive, so they have become inactive after sitting in our cold room. I will get a new plate from Eric tomorrow, then grow it in our lab and make stock. I also plan to make more TY liqud media today.
