Team:UNITN-Trento

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             <p>Our team is proud to introduce to you <i>B. fruity</i>, a new environment-friendly way to control fruit ripening by exploiting an engineered light-regulated strain of <i>B. subtilis</i>. The system works by synthesizing ethylene or methyl salicylate (MeSA) upon photoinduction.</p>
             <p>Our team is proud to introduce to you <i>B. fruity</i>, a new environment-friendly way to control fruit ripening by exploiting an engineered light-regulated strain of <i>B. subtilis</i>. The system works by synthesizing ethylene or methyl salicylate (MeSA) upon photoinduction.</p>
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             <p><b>Ethylene</b> is a plant hormone widely used to ripen fruit such as bananas, kiwifruit, apples, and others. However: synthesis, handling, and storage of ethylene is expensive and dangerous. In contrast, <i>B. fruity</i> produces ethylene from inexpensive material by hijacking a metabolic intermediate from the TCA cycle (2-oxoglutarate). It converts this metabolite to ethylene throught the activity of an ethylene forming enzyme (EFE, 2-oxoglutarate oxygenase/decarboxylase) from <i>Pseudomonas syringae</i>.</p>
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             <p><b>Ethylene</b> is a plant hormone widely used to ripen fruit. However its synthesis, handling, and storage is expensive and dangerous. In contrast, <i>B. fruity</i> produces ethylene from inexpensive material by hijacking a metabolic intermediate, 2-oxoglutarate. Our system converts this metabolite to ethylene through the activity of an ethylene forming enzyme (EFE, 2-oxoglutarate oxygenase/decarboxylase) from <i>Pseudomonas syringae</i>.</p>
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             <p><i>B. fruity</i> does not just accelerate ripening, but can also slow the process down, when desired, through the incorporation of a <b>methyl salicylate</b> (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwi and tomatoes. The explored MeSA pathway builds upone the 2006 MIT iGEM project “<i>Eau de coli</i>”.</p>
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             <p><i>B. fruity</i> does not just accelerate ripening, but can also slow the process down, when desired, through the incorporation of a <b>methyl salicylate</b> (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwifruit and tomatoes. The explored MeSA pathway builds upon the 2006 MIT iGEM project “<i>Eau de coli</i>”.</p>
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             <p>As a proof of concept, we engineered <i>E. coli</i> with the above systems plus the YF1/FixJ blue light receptor device.</p>
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             <p>As a proof of concept, we engineered <i>E. coli</i> with the above system plus the YF1/FixJ blue light receptor device.</p>
             <p>We are hopeful that <i>B. fruity</i> will simplify the process of bringing fresh fruit from the field to the consumer.</p>
             <p>We are hopeful that <i>B. fruity</i> will simplify the process of bringing fresh fruit from the field to the consumer.</p>

Revision as of 14:50, 27 September 2013


Achievements

  • Built and submitted XX New BioBricks;
  • Successfully characterized YY BioBricks;
  • Improved XX parts;
  • Hosted a high school student in the lab for the summer;
  • Asked the opinion of local fruit consumers on our project;
  • Designed a fruit vending machine and an home edition B. fruity products;
  • Ripened XX bananas, YY tomatoes, zz kiwis, UU plums;
  • Eaten one banana bread, one plums crumble and one apple strudel.
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