Team:Paris Bettencourt/Results

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Background

CRISPR/Cas systems generate site-specific double strand breaks and have recently be used for genome editing.

Results

  • Successfully cloned gRNA anti-KAN, crRNA anti-KAN, tracrRNA-Cas9 and pRecA-LacZ into Biobrick backbones and therefore generated four new BioBricks.
  • Testing the new assembly standard for our cloning.

BioBricks

  1. BBa_K1137012 (gRNA anti-KAN)
  2. BBa_K1137013 (crRNA anti-KAN)
  3. BBa_K1137014 (tracrRNA-Cas9)
  4. BBa_K1137015 (pRecA-LacZ)

Aims

Building a genotype sensor based on CRISPR/Cas that reports existance of an antibiotic resistance gene.

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
team2013@igem-paris.org
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