User contributions
From 2013.igem.org
- 15:43, 4 October 2013 (diff | hist) Team:Leicester/Human Practises
- 15:41, 4 October 2013 (diff | hist) N Team:Leicester/Human Practises (Created page with "==Human Practises==")
- 15:39, 4 October 2013 (diff | hist) Team:Leicester/Attributions
- 15:38, 4 October 2013 (diff | hist) Team:Leicester/Attributions
- 15:37, 4 October 2013 (diff | hist) Team:Leicester/Safety
- 15:37, 4 October 2013 (diff | hist) Team:Leicester/Notebook
- 15:37, 4 October 2013 (diff | hist) Team:Leicester/Parts
- 15:36, 4 October 2013 (diff | hist) Team:Leicester/Project
- 15:36, 4 October 2013 (diff | hist) Team:Leicester/Team (top)
- 15:35, 4 October 2013 (diff | hist) Team:Leicester/Team
- 15:34, 4 October 2013 (diff | hist) Team:Leicester/Modeling (top)
- 15:33, 4 October 2013 (diff | hist) Team:Leicester/Modeling
- 15:29, 4 October 2013 (diff | hist) Team:Leicester/Modeling
- 15:28, 4 October 2013 (diff | hist) Team:Leicester/Modeling
- 15:27, 4 October 2013 (diff | hist) Team:Leicester/Modeling
- 15:23, 4 October 2013 (diff | hist) Team:Leicester/Modeling
- 15:21, 4 October 2013 (diff | hist) Team:Leicester/Modeling
- 15:20, 4 October 2013 (diff | hist) Team:Leicester/Modeling
- 15:18, 4 October 2013 (diff | hist) Team:Leicester
- 15:17, 4 October 2013 (diff | hist) Team:Leicester/Modeling
- 10:03, 20 September 2013 (diff | hist) N 20/09/13 (Created page with "==Miniprep from overnight broths== *DNA is isolated using Omega Miniprep kit and its protocol is followed ==Nanodropping the isolated DNA== {|border=1 |Sample||Volume ul||Concen...")
- 09:59, 20 September 2013 (diff | hist) Team:Leicester/Notebook
- 09:59, 20 September 2013 (diff | hist) 18/09/13 (→Plating out TOD operon bacteria) (top)
- 09:58, 20 September 2013 (diff | hist) 19/09/13
- 09:57, 20 September 2013 (diff | hist) 19/09/13
- 10:45, 18 September 2013 (diff | hist) N 18/09/13 (Created page with "==Making and running an agarose gel== *Making a 3% agarose gel to run Tod PCR fusion genes from 17/09 *This is to determine that the digestion works *Running the gel with digeste...")
- 10:41, 18 September 2013 (diff | hist) 17/09/13 (→Ligation of Tod genes and pSB1C3 backbone)
- 10:37, 18 September 2013 (diff | hist) 17/09/13 (→Ligation of Tod genes and pSB1C3 backbone)
- 10:37, 18 September 2013 (diff | hist) 17/09/13 (→Ligation of Tod genes and pSB1C3 backbone)
- 10:37, 18 September 2013 (diff | hist) 17/09/13 (→Ligation of Tod genes and pSB1C3 backbone)
- 10:36, 18 September 2013 (diff | hist) 17/09/13 (→Digestion of new fusion PCR Tod genes)
- 10:23, 18 September 2013 (diff | hist) 17/09/13 (→Digestion of new fusion PCR Tod genes)
- 10:16, 18 September 2013 (diff | hist) 17/09/13 (→Results from previous day)
- 10:08, 18 September 2013 (diff | hist) N 17/09/13 (Created page with "==Results from previous day== *Background control plate had growth, which was not expected. This suggests that pSB1C3 backbone religated. *It was also thought that the Tod genes...")
- 10:02, 18 September 2013 (diff | hist) Team:Leicester/Notebook
- 14:52, 16 September 2013 (diff | hist) 16/09/13 (→Digestion of the pGEM plasmid gel extracted on the 13/09/13)
- 14:52, 16 September 2013 (diff | hist) 16/09/13 (→Digestion of the pGEM plasmid extracted on the 13/09/13)
- 14:08, 16 September 2013 (diff | hist) 16/09/13 (→Running the backbone and RFP on gel)
- 14:05, 16 September 2013 (diff | hist) 16/09/13 (→Ligation of digested pSB1C3 backbone to Tod F, X and Tob G)
- 14:04, 16 September 2013 (diff | hist) 16/09/13 (→Transformation of TOD genes ligations done on the 02/09/13 using the pGEM-T Vector System I-A3600 (changes were made to the protocol))
- 14:02, 16 September 2013 (diff | hist) 16/09/13 (→Ligation of digested pSB1C3 backbone to Tod F, X and Tob G)
- 14:01, 16 September 2013 (diff | hist) 16/09/13 (→Ligation of digested pSB1C3 backbone to Tod F, X and Tob G)
- 14:01, 16 September 2013 (diff | hist) 16/09/13
- 12:30, 16 September 2013 (diff | hist) 16/09/13
- 12:27, 16 September 2013 (diff | hist) 16/09/13 (→Digestion of the TOD genes PCR products)
- 12:26, 16 September 2013 (diff | hist) 16/09/13 (→Digestion of the TOD genes PCR products)
- 12:18, 16 September 2013 (diff | hist) 16/09/13 (→Digestion of the TOD genes PCR products)
- 12:13, 16 September 2013 (diff | hist) 16/09/13
- 11:21, 16 September 2013 (diff | hist) 16/09/13 (→Digesting pSB1C3 backbone)
- 11:20, 16 September 2013 (diff | hist) N 16/09/13 (Created page with "==Digesting pSB1C3 backbone== *Digesting for ligation with the already digested Tod operon PCR products *Following the iGEM official protocol *Using EcoRI-HF and PstI-HF *Exludin...")
- 11:14, 16 September 2013 (diff | hist) Team:Leicester/Notebook
- 10:23, 30 August 2013 (diff | hist) 30/08/13 (→Repeating the combustion experiment)
- 10:22, 30 August 2013 (diff | hist) N 30/08/13 (Created page with "==Preparing samples to be sent to be sequenced by PNACL== ==Repeating the combustion experiment== *Blocks of both DNA and no DNA coating were made *Block thicker than last experi...")
- 10:20, 30 August 2013 (diff | hist) Team:Leicester/Notebook
- 10:19, 30 August 2013 (diff | hist) 29/08/13
- 14:02, 29 August 2013 (diff | hist) 31/07/13 (→Results from the Transformation of limonene Biobrick (BBa_K118025) to the pSB1C3 vector) (top)
- 14:01, 29 August 2013 (diff | hist) 28/08/13 (→DNA extraction from gel) (top)
- 14:01, 29 August 2013 (diff | hist) 28/08/13 (→DNA extraction from gel)
- 14:00, 29 August 2013 (diff | hist) 29/08/13 (→Run Tod operon ligation genes on the gel)
- 13:59, 29 August 2013 (diff | hist) N 29/08/13 (Created page with "==Make 2% agarose gel== ==Run Tod operon ligation genes on the gel== ==Isolate the DNA from gel== *DNA isolated using Bioline Isolate II PCR and gel kit and following its protocol")
- 13:57, 29 August 2013 (diff | hist) Team:Leicester/Notebook
- 13:55, 29 August 2013 (diff | hist) 28/08/13 (→gel electrophoresis)
- 11:43, 28 August 2013 (diff | hist) Team:Leicester/Notebook
- 11:35, 23 August 2013 (diff | hist) N File:Igem ovenight incub herrinngDNA 230813.tif (top)
- 11:34, 23 August 2013 (diff | hist) 23/08/13 (→Running overnight dilutions on gel)
- 11:34, 23 August 2013 (diff | hist) 23/08/13
- 11:15, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm DNA)
- 11:14, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:14, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:14, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:10, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:10, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:09, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:07, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:06, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:06, 23 August 2013 (diff | hist) 23/08/13 (→Nanodropping overnight incubations of herring sperm)
- 11:05, 23 August 2013 (diff | hist) N 23/08/13 (Created page with "==Nanodropping overnight incubations of herring sperm== *Table representing the concentrations of herring sperm after and before different treatments {|border=1 |Sample||Concentr...")
- 09:19, 23 August 2013 (diff | hist) Team:Leicester/Notebook
- 09:07, 23 August 2013 (diff | hist) 22/08/13 (→Running sonicated herring sperm DNA on 0.8% gel)
- 09:03, 23 August 2013 (diff | hist) N File:Igem shear son 220813.jpg (top)
- 09:03, 23 August 2013 (diff | hist) 22/08/13 (→Running sonicated herring sperm DNA on 0.8% gel)
- 12:42, 21 August 2013 (diff | hist) Team:Leicester/Notebook
- 12:42, 21 August 2013 (diff | hist) 20/08/13 (→Plating out bacteria)
- 12:41, 21 August 2013 (diff | hist) 20/08/13 (→Plating out bacteria)
- 12:41, 21 August 2013 (diff | hist) N 20/08/13 (Created page with "==Making nutrient agar plates and broth== *Making up broth and agar solution to grow ''P. putida'' in. *For agar plates: **9.2g of nutrient agar medium **400ml of distilled water...")
- 14:16, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 14:02, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 14:02, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 13:58, 19 August 2013 (diff | hist) N File:Igem denatured son 190813.jpg (top)
- 13:57, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 11:04, 19 August 2013 (diff | hist) 19/08/13 (→Running both denatured and sonicated samples on gel)
- 11:04, 19 August 2013 (diff | hist) N 19/08/13 (Created page with "==Denaturing sonicated DNA== *Denaturing DNA by heat shock for 10mins in a 95C heat block *Adding 5ul of OG dye ==Running both denatured and sonicated samples on gel== *For just...")
- 11:01, 19 August 2013 (diff | hist) Team:Leicester/Notebook
- 13:40, 16 August 2013 (diff | hist) 16/08/13 (→Running the gel from sonication series)
- 13:39, 16 August 2013 (diff | hist) N File:Igem 2nd son series 160813.jpg (top)
- 13:39, 16 August 2013 (diff | hist) N 16/08/13 (Created page with "==Running the gel from sonication series== *Samples were taken from sonicated tube every 30min intervals, a total of 6 samples. *Each was diluted 100 fold to get a concentration ...")
- 13:34, 16 August 2013 (diff | hist) Team:Leicester/Notebook
- 09:20, 16 August 2013 (diff | hist) 18/07/13 (Replaced content with "==Double digest==")
- 12:01, 12 August 2013 (diff | hist) 12/08/13 (→Running an agarose gel for sonication analysis)
- 11:50, 12 August 2013 (diff | hist) 12/08/13 (→Running an agarose gel for sonication analysis)
- 11:50, 12 August 2013 (diff | hist) 12/08/13 (→Running an agarose gel for sonication analysis)
- 11:49, 12 August 2013 (diff | hist) 12/08/13 (→Sonicating herring sperm DNA)
- 11:49, 12 August 2013 (diff | hist) N 12/08/13 (Created page with "==Sonicating herring sperm DNA== *Pipetting out sonicated samples every 30mins **Pipette 4ul of DNA into Son 1-6 every 30mins ***Dilute 1ul from Son 1-6 in 9ul of 1xTE buffer to ...")
- 11:34, 12 August 2013 (diff | hist) Team:Leicester/Notebook
- 09:30, 12 August 2013 (diff | hist) 09/08/13 (→Glycerol stocks)
- 09:29, 12 August 2013 (diff | hist) 09/08/13 (→Running an agarose gel)
- 16:33, 9 August 2013 (diff | hist) N File:Lim chlorodig sacI 090813.jpg (top)
- 16:33, 9 August 2013 (diff | hist) 09/08/13 (→Running an agarose gel)
- 16:00, 9 August 2013 (diff | hist) 09/08/13 (→Sonicating Herring sperm DNA)
- 15:28, 9 August 2013 (diff | hist) 09/08/13 (→Running an agarose gel)
- 10:15, 7 August 2013 (diff | hist) 07/08/13
- 10:06, 7 August 2013 (diff | hist) N 07/08/13 (Created page with "{|border=1 |Sample||Concentration||260/280||260/230 |- |1kb||534||1.82||1.95 |- |1||52.7||1.77||2.05 |- |2||4.6||1.46||1.88 |- |3||0.1||0.24||-0.29 |- |Overnight |- |1in 10||24.8...")
- 08:35, 7 August 2013 (diff | hist) Team:Leicester/Notebook
- 10:03, 6 August 2013 (diff | hist) 06/08/13 (→Running of an agarose gel)
- 16:30, 31 July 2013 (diff | hist) Team:Leicester
- 15:41, 31 July 2013 (diff | hist) N File:Lim1 digest 31 07 2013.jpg (The bands are well separated as shown by the middle 4 lanes, the two outermost lanes are the markers. 5um of 1kb ladder was used as a marker for each of the two lanes and 10um of orange G loading dye was added to the samples.) (top)
- 15:37, 31 July 2013 (diff | hist) 31/07/13 (→Results from the Transformation of limonene Biobrick (BBa_K118025) to the pSB1C3 vector)