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From 2013.igem.org

Revision as of 23:12, 27 September 2013 (view source) Mague (Talk | contribs) ← Older edit		Latest revision as of 02:57, 28 September 2013 (view source) Gabyqz (Talk | contribs)
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	[[File:Piezasgens.jpg|right|300px]]		[[File:Piezasgens.jpg|right|300px]]
-		+	Plasmid extraction from synthetized parts
	pUC57: 2710 bp		pUC57: 2710 bp
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-	Pieces clonning in pSB1C3: 2070 bp	+	Parts clonning in pSB1C3: 2070 bp
-	In the lane 1 is the ladder of 3000 bp	+	*In the lane 1 is the ladder of 3000 bp
-	The sample of the lane 3 is the promoter 2	+	*The sample of the lane 3 is the promoter 2
-	The sample of the lane 4 is the promoter 1	+	*The sample of the lane 4 is the promoter 1
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-	The sample of the lane 7 is the RBS	+
		+	*The sample of the lane 7 is the RBS
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-	Plasmid with GFP	+	Ligation Promoters with GFP
-	In the lane 1 is the ladder of 5000 bp	+	*In the lane 1 is the ladder of 5000 bp
-	The sample of the lane 3 is the Plasmid GFP	+	*The sample of the lane 3 is the Plasmid GFP
-	The sample of the lane 5 is the Plasmid GFP	+	*The sample of the lane 5 is the Plasmid GFP
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		+	----
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		+	[[File:P1_expression.jpg|center|300px]]
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		+	GFP expression for L.Plantarum promoter cloned in E.coli
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		+	----
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		+	[[File:P2_expression.jpg|center|300px]]
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		+	GFP expression for L.Plantarum promoter 2 cloned in E.coli

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Latest revision as of 02:57, 28 September 2013

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Results

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Plasmid extraction from synthetized parts

pUC57: 2710 bp

• In the lane 1 is the ladder of 5000 bp

• The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp

• The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp

• The sample of the lane 7 is the RBS, inserted sequence: 76 bp

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Parts clonning in pSB1C3: 2070 bp

• In the lane 1 is the ladder of 3000 bp

• The sample of the lane 3 is the promoter 2

• The sample of the lane 4 is the promoter 1

• The sample of the lane 7 is the RBS

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Ligation Promoters with GFP

• In the lane 1 is the ladder of 5000 bp

• The sample of the lane 3 is the Plasmid GFP

• The sample of the lane 5 is the Plasmid GFP

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GFP expression for L.Plantarum promoter cloned in E.coli

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GFP expression for L.Plantarum promoter 2 cloned in E.coli