Team:BYU Provo/Notebook/Phage Purification/Springexp

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Phage Purification May - June Notebook



Overview
March-April
May-June
July-August
September-October

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May 1 - May 12


This semester we will are perfecting our procedures of purifying T4 and T7 bacteriophage through use of PEG and a CsCl gradient. During this week we began growing cultures to use and infect with phage to begin purification. We were held back waiting for materials, but were able to plan for our future procedures.


Daily log

Experiment Listing

Progress Report


Spring1.JPG


May 13 - May 26


In these two weeks, we performed our first round of mutagenesis and first PCR. Although we are still testing the results to optimize our protocol, we are optimistic about the outcome!


Daily log

Experiment Listing

Progress Report



Spring2.JPG


May 27 - June 9


We finished our CsCl gradient and have started a new round of phage purification to run through the CsCl gradient again.


Daily log

Experiment Listing

Progress Report



Spring3.JPG


June 10 - June 23


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Daily log

Experiment Listing

Progress Report



Spring4.JPG
June 24 - June 30


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Daily log

Experiment Listing

Progress Report


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Winter2.JPG