Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Exp/7.27 Bacteria Growth Curve After Dilution
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* Pure LB was used to as blank | * Pure LB was used to as blank | ||
* 1mL of E coli solution at various concentration and time points was pipetted into each cuvette for measurement. | * 1mL of E coli solution at various concentration and time points was pipetted into each cuvette for measurement. | ||
- | ** The time points include 0, 0.5, 1, 1.5, 2, 2.5, 3.5 | + | ** The time points include 0, 0.5, 1, 1.5, 2, 2.5, and 3.5 hours |
Revision as of 14:46, 29 July 2013
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7.27 Bacteria Growth Curve After Dilution
I) Purpose
II) Expected Outcome
III) Reagants Used
1. Bacteria preparation
2. Measurements using spectrophotometer
1. Raw data OD600 of the BL21 liquid culture overnight was 1.063. Surprisingly, this is different than our results from 5.13 Determining E coli Concentration With Spectrophotometer. This implies that we'll have to take several more measurements with overnight liquid culture to determine whether the concentration of bacteria in liquid culture is stable.
2. Plot
3. Linear Regression Result
VI) Conclusion
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