Team:BYU Provo/Notebook/SmallPhage/Summerexp/Period2/Exp/7.27 Bacteria Growth Curve After Dilution
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'''I) Purpose''' | '''I) Purpose''' | ||
* Determine the growth curve of bacteria after dilution. This information will be used to estimate the amount of bacterial growth during incubation in mutagenesis experiments. | * Determine the growth curve of bacteria after dilution. This information will be used to estimate the amount of bacterial growth during incubation in mutagenesis experiments. | ||
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'''II) Expected Outcome''' | '''II) Expected Outcome''' | ||
* A sigmoidal growth curve for each dilution. | * A sigmoidal growth curve for each dilution. | ||
* The bigger the initial dilution factor, the longer it takes for bacteria to reach its growth plateau. | * The bigger the initial dilution factor, the longer it takes for bacteria to reach its growth plateau. | ||
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'''III) Reagants Used''' | '''III) Reagants Used''' | ||
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2. Plot | 2. Plot | ||
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'''VI) Conclusion''' | '''VI) Conclusion''' | ||
+ | : This experiment indicates that BL21 overnight liquid culture might not have the same concentration of bacteria. We'll need to measure bacterial concentration using spectrophotometer every time during mutagenesis to get an accurate estimate of the number of bacteria we are adding. However, to get approximately OD 0.5, for our dilution step in our mutagenesis protocol, we will use 1:50. | ||
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Revision as of 21:50, 29 July 2013
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7.27 Bacteria Growth Curve After Dilution
I) Purpose
1. Bacteria preparation
2. Measurements using spectrophotometer
1. Raw data OD600 of the BL21 liquid culture overnight was 1.063. Surprisingly, this is different than our results from 5.13 Determining E coli Concentration With Spectrophotometer. This implies that we'll have to take several more measurements with overnight liquid culture to determine whether the concentration of bacteria in liquid culture is stable.
2. Plot VI) Conclusion
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