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Team:Imperial College/Protocols
From 2013.igem.org
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<b>Waste Conditioned Media (WCM)</b> | <b>Waste Conditioned Media (WCM)</b> | ||
<p>We took 6g SRF (Solid Recovered Fuel) and to this added 300 mL LB. In addition to this we prepared 300 mL LB. Post-autoclave, the SRF LB was sterile filtrated into 50 mL falcons, which we will now call Waste Conditioned Media (WCM). To both the LB and WCM, 150 µl of chloramphenicol was added. 100 mL WCM was added to 3 pre-sterilised flasks, this was repeated for LB.</p> | <p>We took 6g SRF (Solid Recovered Fuel) and to this added 300 mL LB. In addition to this we prepared 300 mL LB. Post-autoclave, the SRF LB was sterile filtrated into 50 mL falcons, which we will now call Waste Conditioned Media (WCM). To both the LB and WCM, 150 µl of chloramphenicol was added. 100 mL WCM was added to 3 pre-sterilised flasks, this was repeated for LB.</p> | ||
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<p>1mL stress response transformed (BBa_K639003) MG1655 grown overnight was added to each of the flasks. This was placed on a shaking incubator at 37°C. T=0 was taken after an hour for the bacteria to normalise to their environment. After this time points were taken every 6 or 12h. </p> | <p>1mL stress response transformed (BBa_K639003) MG1655 grown overnight was added to each of the flasks. This was placed on a shaking incubator at 37°C. T=0 was taken after an hour for the bacteria to normalise to their environment. After this time points were taken every 6 or 12h. </p> | ||
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Revision as of 17:58, 20 August 2013
Protocols
Glucose Assay
Waste Conditioned Media (WCM)
We took 6g SRF (Solid Recovered Fuel) and to this added 300 mL LB. In addition to this we prepared 300 mL LB. Post-autoclave, the SRF LB was sterile filtrated into 50 mL falcons, which we will now call Waste Conditioned Media (WCM). To both the LB and WCM, 150 µl of chloramphenicol was added. 100 mL WCM was added to 3 pre-sterilised flasks, this was repeated for LB.
1mL stress response transformed (BBa_K639003) MG1655 grown overnight was added to each of the flasks. This was placed on a shaking incubator at 37°C. T=0 was taken after an hour for the bacteria to normalise to their environment. After this time points were taken every 6 or 12h.
