Team:KIT-Kyoto/Notebook/ATF2

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Latest revision as of 02:26, 28 September 2013

ATF2

10th August

We performed PCR to amplify the ATF2 gene.

F:3' CGATCCCATGGGGATGGAAGATATAGAAGGATACGAACCACA

R:5' CGATCGGATCCAAAAGCGACGCAAATTCGCCGATGGTTTG

Buffer	50µL
dNTP	20µL
Primer mix	1µL
DNA sample	0.5µL
KOD-FX	2µL
H₂O	26.5µL
Total	100µL

PCR products were electrophoresed in 1% agarose gel.

We detected a DNA band at around 1600bp.

PCR reactions were carried out (total volume was 300µL).

PCR products were electrophoresed in 1% agarose gel.

The PCR product with the size (1.6 kb) was verified as ATF2.

The PCR products of ATF2 was purified by ethanol precipitation

12th August

The PCR product of ATF2 were digested with the XhoI.

ATF2	5µL
Buff	2µL
H₂O	12µL
Xho1	1µL

No DNA was detected.

We have repeated the PCR reactions to amplify ATF2 under the same conditions as those carried out on 10th, August.

14th August

1.

PCR reaction was carried out by using the following primer for pSB1C3.

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG

PCR products were electrophoresed in 1% agarose gel.

The PCR product with the size (1.6 kb) was verified as ATF2.

The PCR products of ATF2 was purified by ethanol precipitation.

2.

The ATF2 gene and pET15b were purified by ethanol precipitation.

<i>ATF2</i> was digested with the XhoI for verification.

ATF2	5µL
Buff	2µL
H₂O	12µL
Xho1	1µL

PCR products were electrophoresed in 1% agarose gel.

The PCR products with the expected size were detected.

ATF2 treated ethanol precipitation and pET-15b was digested with the NcoI and BamHI.

pET-15b	87µL
Nco1	1µL
BamH1	1µL
Buff.	10µL
BSA	1µL
(total 100µL)

ATF2	24µL
Nco1	1µL
BamH1	1µL
Buff.	3µL
BSA	1µL
(total 30µL)

It was electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

17th August

pET-15b was digested with the Nco1 and BamH1.

pET-15b	87µL
Nco1	1µL
BamH1	1µL
Buff.	10µL
BSA	1µL
(total 100µL)

We treated the vector with 1µL BAP for 30 minutes.

It was electrophoresed in 1% Blue gel

ATF2 were extracted from the Blue gel

19th August

The ATF2 gene and pET-15b were purified.

We ligated pET-15b and ATF2.

ATF2	5µL
pET-15b	5µL
L.MIX	10µL
total 20µL

After the ligatation, we have transformed them into E.coli cells.

20th August

We got approximately 70 colonies and cultured them furthermore.

21th August

We checked the insertion of DNA by colony cracking.

We could see correct bands of plasmid DNA only from five samples.

We cultured each transformant in LB liquid medium containing ampicillin.

PCR reaction was carried out using the following primers

1-1

F:3' TCGGCAATGCACAGTCATGGCCATAGTAGA

R:5' TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT

1-2

F:3' CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA

R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

2.

PCR products were electrophoresed in 1% agarose gel.

The PCR products with the size (1.6 kb) were verified as ATF2.

The PCR products of ATF2 were purified by ethanol precipitation and digested with the following composition.

1-1

ATF2	25µL
Mfe1	2µL
Buff.	3µL
Total 30µL

1-2

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It were electrophoresed in 1% Blue gel

ATF2 were extracted from the Blue gel

22th August

I

1

ATF2 gene extracted from the Blue gel was treated ethanol precipitation.

2

Ligation of 2-1 and 2-2 was performed by using Ligation mix.

1-1 with 1-2 and 2-1 with 2-2

The PCR products of ATF2 gene was purified.

It was electrophoresed in 1% agarose gel.

No DNA was detected.

II

1

PCR reaction was carried out using the primers.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

1-1

ATF2	25µL
Mfe1	2µL
Buff.	3µL
Total 30µL

1-2

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% Blue gel.

Any DNA fragment was not detected.

III

PCR reaction was carried out using the following primers.

1-1

F:3' TCGGCAATGCACAGTCATGGCCATAGTAGA

R:5' TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT

1-2

F:3' CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA

R:5'

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

1-1

ATF2	25µL
Mfe1	2µL
Buff.	3µL
Total 30µL

1-2

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

It were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

IV

PCR reaction was carried out using the primer.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

1-1

ATF2	25µL
Mfe1	2µL
Buff.	3µL
Total 30µL

1-2

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

23th August

The plasmids (d, e, f, I, o) were digested with Nco1 and BamH1.

We applied them to 1% agarose gel electrophoresis and checked them.

Sample d, e, f, i had two bands and we cultured them.

The plasmids (d, e, f, I,) were digested with BsiWI and checked.

We detected the correct size band.

We minipreped and transformed them into BL21(DE3).

I

Sample 1.

ATF2 gene (Ⅳ) extracted from the Blue gel was purified by ethanol precipitation.

Sample 2.

ATF2 gene (III) extracted from the Blue gel was purified by ethanol precipitation.

Ligation of sample 1 and 2 was performed by Ligation mix.

PCR reaction was carried out using the primers

PCR products were electrophoresed in 1% agarose gel, and several fragments were detected.

II

PCR reaction was carried out using the primers

1-1

F:3' TCGGCAATGCACAGTCATGGCCATAGTAGA

R:5' TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT

1-2

F:3' CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA

R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG

The PCR products of ATF2 gene were purified, and digested with Mfe and EcoRI.

After the digestion, the ATF2 gene was electrophoresed in 1% Blue gel.

The ATF2 genes were extracted from the Blue gel and purified.

Ligation of 2-1 and 2-2 was performed using Ligation mix.

PCR reaction was carried out using the primers

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG

24th August

I

PCR reaction was carried out using the following primers.

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% Blue gel.

No DNA was detected.

II

１

PCR react ion was carried out using the primer.

The PCR products for ATF2 was treated ethanol precipitation.

It was digested with the following composition.

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

Ligation of 2-1 and 2-2 was performed using Ligation mix.

III

1.

PCR reaction was carried out using the primer.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% agarose gel.

Multiple DNA fragments were detected.

IV

１

PCR reaction was carried out using the primers.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

ATF2	25µL
EcoR1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% agarose gel.

Multiple DNA fragments were detected for the DIAP2.

26th August

Cell free extract was prepared and applied to SDS-PAGE to detect ATF2 protein.

27th August

PCR products were electrophoresed in 1% agarose gel.

The PCR products were treated ethanol precipitation.

They were digested.

They were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was treated ethanol precipitation.

Ligation of 2-1 and 2-2 was performed using Ligation mix.

We purified them.

28th August

The PCR products was treated ethanol precipitation.

PCR reaction was carried out by using the primer.

PCR products were electrophoresed in 1% agarose gel.

No DNA was detected.

We performed PCR, purified, and digested again.

We ligated and purified them.

29th August

I

PCR reaction was carried out using the primer.

PCR products were electrophoresed in 1% agarose gel.

No DNA band was detected.

II

PCR reaction was carried out using the following primers.

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

The PCR products were treated ethanol precipitation.

It was digested with the following composition.

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

We ligated them and purified it.

We performed PCR to amplify it.

Cell free extract was prepared with FastBreak Cell Lysis Reagent and applied to SDS-PAGE.

30th August

I

1

PCR reaction was carried out using the following primers

1-1

F:3' TCGGCAATGCACAGTCATGGCCATAGTAGA

R:5' TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT

1-2

F:3' CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA

R:5'

The PCR products were purfied.

It was digested with the following composition.

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It were electrophoresed in 1% Blue gel.

ATF2 were extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

We ligated them and purified it.

PCR reaction was carried out using the following primers for pSB1C3.

PCR products were electrophoresed in 1% agarose gel.

The PCR products of ATF2 gene was purified.

It was digested with the following composition.

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

PCR products were electrophoresed in 1% agarose gel.

The PCR products of ATF2 gene was purified.

II

１

We performed PCR (prepared 8/29).

PCR reaction was carried out using the following primers.

2-1

F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT

R:5' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

2-2

F:3' CGCAGTCTGCAGGCGCCTTGAGATTTGCGTTCGGCCTAA

R:5' ATCATCTTCGTAATCCTTGATGTGAATAGTGC

The PCR products were purified.

It was digested with the following composition.

2-1

ATF2	25µL
Nsi1	2µL
Buff.	3µL
Total 30µL

2-2

ATF2	25µL
Pst1	2µL
Buff.	3µL
Total 30µL

It was electrophoresed in 1% Blue gel.

ATF2 was extracted from the Blue gel.

ATF2 extracted from the Blue gel was purified.

We ligated it.

We purified it and performed PCR.

2^nd September

We purified the mutant of ATF2.

We digested it and cc3 with Xba1 and Spe1.

We electrophoresed and extracted them in and from 1% Blue gel.

We purified them.

We ligated and transformed them into E.coli cells.

4^th September

We purified the mutant of ATF2.

Three tubes of pSB1C3 were treated ethanol precipitation

We add 25mL H₂O and digested it with Xba1 and Spe1.

We electrophoresed and extracted them in and from 1% Blue gel.

We purified them.

We ligated and transformed them into E.coli cells.

5^th September

We did rapid check of the insert by colony cracking (ATF2+pSB1C3).

But we detected no band.

We cultured them for 5 hours and minipreped.

We checked them by electrophoresing but couldn’t see any bands.

So, we thought them failed.

We cultured other sample (ATF2+pSB1C3).

@@ Line 5: / Line 5: @@
 <head>
 </head>
+</html>
 <div class="document">
    <div id="doc-left">
-<ul class="nav metro-nav-list">
+{{Template:KIT-Kyoto/menu_note}}
-   <li class="nav-header">LabNote</li>
-   <li class="">
-      <a href="#ATF1">ATF1</a>
-      <ul class="nav">
-          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/may">May</a></li>
-          <li><a href="">june</a></li>
-          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/july">july</a></li>
-          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/august">August</a></li>
-          <li><a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF1/september">September</a></li>
-      </ul>
-   </li>
-   <li class="">
-      <a href="#https://2013.igem.org/Team:KIT-Kyoto/Notebook/ATF2">ATF2</a>
-      <ul class="nav">
-      </ul>
-   </li>
-   <li class="">
-      <a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/YJL">YJL068C</a>
-      <ul class="nav">
-      </ul>
-   </li>
-   <li class="">
-      <a href="https://2013.igem.org/Team:KIT-Kyoto/Notebook/growcurves">Growth Curves</a>
-      <ul class="nav">
-      </ul>
-   </li>
-  </ul>
    </div>
 <div id="doc-right" class="metro">
@@ Line 50: / Line 16: @@
 <h2>ATF2</h2>
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>10th August</span></b></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We performed PCR
-to amplify the <span class=MsoSubtleEmphasis><span style='color:black'>ATF2</span></span><span
+to amplify the <span class=MsoSubtleEmphasis><span style='color:black'><i>ATF2</i></span></span><i><span
-style='color:black'> g</span>ene.</span></p>
+style='color:black'> </span></i><span style='color:black'>g</span>ene.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3'
@@ Line 77: / Line 38: @@
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>50uL</span></p>
+   style='font-size:11.0pt'>50&micro;L</span></p>
    </td>
   </tr>
@@ Line 90: / Line 51: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>20uL</span></p>
+   style='font-size:11.0pt'>20&micro;L</span></p>
    </td>
   </tr>
@@ Line 103: / Line 64: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.9pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>1uL</span></p>
+   style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 116: / Line 77: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>0.5uL</span></p>
+   style='font-size:11.0pt'>0.5&micro;L</span></p>
    </td>
   </tr>
@@ Line 129: / Line 90: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>2uL</span></p>
+   style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 142: / Line 103: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.9pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>26.5uL</span></p>
+   style='font-size:11.0pt'>26.5&micro;L</span></p>
    </td>
   </tr>
@@ Line 155: / Line 116: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:9.7pt'>
    <p class=MsoListParagraph style='margin-left:0mm'><span lang=EN-US
-   style='font-size:11.0pt'>100uL</span></p>
+   style='font-size:11.0pt'>100&micro;L</span></p>
    </td>
   </tr>
@@ Line 167: / Line 128: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reactions were
-carried out (total volume was 300uL).</span></p>
+carried out (total volume was 300&micro;L).</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR products were
@@ Line 174: / Line 135: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR product
 with the size (1.6 kb) was verified as <span class=MsoSubtleEmphasis><span
-style='color:windowtext'>ATF2.</span></span></span></p>
+style='color:windowtext'><i>ATF2</i>.</span></span></span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products of
-<span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2</span></span>
+<span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i></span></span>
 was purified by ethanol precipitation</span></p>
@@ Line 187: / Line 148: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR product of
-<span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2</span></span>
+<span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i></span></span>
 were digested with the <span class=MsoSubtleEmphasis><span style='color:windowtext'>Xho</span></span>I.</span></p>
@@ Line 196: / Line 157: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:17.1pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:windowtext'>ATF2</span></span></p>
+   style='font-size:11.0pt;color:windowtext'><i>ATF2</i></span></span></p>
    </td>
    <td width=61 valign=top style='width:46.1pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:17.1pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5&micro;L</span></p>
    </td>
   </tr>
@@ Line 211: / Line 172: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 222: / Line 183: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12&micro;L</span></p>
    </td>
   </tr>
@@ Line 235: / Line 196: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 244: / Line 205: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We have repeated
 the PCR reactions to amplify <span class=MsoSubtleEmphasis><span
-style='color:windowtext'>ATF2</span></span> under the same conditions as those
+style='color:windowtext'><i>ATF2</i></span></span> under the same conditions as those
 carried out on 10th, August.</span></p>
@@ Line 260: / Line 221: @@
 style='font-size:14.0pt'>14th August</span></b></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１．</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 274: / Line 235: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR product
 with the size (1.6 kb) was verified as <span class=MsoSubtleEmphasis><span
-style='color:windowtext'>ATF2.</span></span></span></p>
+style='color:windowtext'><i>ATF2</i>.</span></span></span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2</span></span>
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i></span></span>
 was purified by ethanol precipitation.</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>２．</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The <i>ATF2 </i>gene
 and pET15b were purified by ethanol precipitation.</span></p>
-<p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF2 </span></i><span
+<p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'><i>ATF2</i> </span></i><span
 lang=EN-US style='font-size:11.0pt'>was digested with the <span
 class=MsoSubtleEmphasis><span style='color:windowtext'>Xho</span></span>I for
@@ Line 300: / Line 261: @@
    <td width=61 valign=top style='width:46.1pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:17.1pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5&micro;L</span></p>
    </td>
   </tr>
@@ Line 311: / Line 272: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 322: / Line 283: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>12&micro;L</span></p>
    </td>
   </tr>
@@ Line 335: / Line 296: @@
    border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:17.7pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 347: / Line 308: @@
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:black'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt;color:black'> </span><span lang=EN-US style='font-size:
 .0pt'>treated ethanol precipitation and pET-15b was digested with the <i>Nco</i>I
@@ Line 361: / Line 322: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87&micro;L</span></p>
    </td>
   </tr>
@@ Line 373: / Line 334: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 385: / Line 346: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 396: / Line 357: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
    </td>
   </tr>
@@ Line 407: / Line 368: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 413: / Line 374: @@
    <td width=147 colspan=2 valign=top style='width:110.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:19.55pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100uL)</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100&micro;L)</span></p>
    </td>
   </tr>
@@ Line 426: / Line 387: @@
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span></p>
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>24&micro;L</span></p>
    </td>
   </tr>
@@ Line 442: / Line 403: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 454: / Line 415: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 465: / Line 426: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 476: / Line 437: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 482: / Line 443: @@
    <td width=154 colspan=2 valign=top style='width:115.7pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:19.55pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 30uL)</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 30&micro;L)</span></p>
    </td>
   </tr>
@@ Line 511: / Line 472: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>87&micro;L</span></p>
    </td>
   </tr>
@@ Line 523: / Line 484: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 535: / Line 496: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 546: / Line 507: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.85pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
    </td>
   </tr>
@@ Line 557: / Line 518: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt;height:18.25pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1&micro;L</span></p>
    </td>
   </tr>
@@ Line 563: / Line 524: @@
    <td width=147 colspan=2 valign=top style='width:110.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt;height:19.55pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100uL)</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>(total 100&micro;L)</span></p>
    </td>
   </tr>
@@ Line 569: / Line 530: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We treated the
-vector with 1uL BAP for 30 minutes.</span></p>
+vector with 1&micro;L BAP for 30 minutes.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>It was
@@ Line 601: / Line 562: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5&micro;L</span></p>
    </td>
   </tr>
@@ Line 612: / Line 573: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>5&micro;L</span></p>
    </td>
   </tr>
@@ Line 623: / Line 584: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>10&micro;L</span></p>
    </td>
   </tr>
@@ Line 629: / Line 590: @@
    <td width=147 colspan=2 valign=top style='width:110.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>total 20uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>total 20&micro;L</span></p>
    </td>
   </tr>
@@ Line 653: / Line 614: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&lt;<span
-class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2</span></span></span><span
+class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>+ pET15b&gt;</span></p>
-style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>＋</span><span lang=EN-US
-style='font-size:11.0pt'>pET15b&gt;</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>We checked the
@@ Line 704: / Line 663: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>２</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR products were
@@ Line 714: / Line 673: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products of
-<span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2</span></span>
+<span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i></span></span>
 were purified by ethanol precipitation and digested with the following
 composition.</span></p>
@@ Line 731: / Line 690: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 743: / Line 702: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 754: / Line 713: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 760: / Line 719: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 775: / Line 734: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif";color:black'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 792: / Line 751: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 803: / Line 762: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 809: / Line 768: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 830: / Line 789: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 842: / Line 801: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 853: / Line 812: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 859: / Line 818: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 879: / Line 838: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 891: / Line 850: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 902: / Line 861: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 908: / Line 867: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 914: / Line 873: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>9</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>It were
@@ Line 931: / Line 888: @@
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>22th August</span></b></p>
-<p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span></b></p>
+<p class=MsoNormal><b><span lang=EN-US style='font-size:11.0pt'>I</span></b></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span></p>
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:black'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt;color:black'> </span><span lang=EN-US style='font-size:
 .0pt'>gene extracted from the Blue gel was treated ethanol precipitation.</span></p>
@@ Line 942: / Line 899: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>２</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Ligation of 2-1
@@ Line 951: / Line 908: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products of
-<span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+<span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 962: / Line 919: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅱ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>II</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 970: / Line 927: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 989: / Line 946: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,001: / Line 958: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,012: / Line 969: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,018: / Line 975: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,038: / Line 995: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,050: / Line 1,007: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,061: / Line 1,018: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,067: / Line 1,024: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,088: / Line 1,045: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,100: / Line 1,057: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,111: / Line 1,068: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,117: / Line 1,074: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,132: / Line 1,089: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,149: / Line 1,106: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,160: / Line 1,117: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,166: / Line 1,123: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,179: / Line 1,136: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅲ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>III</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 1,214: / Line 1,171: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,231: / Line 1,188: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,242: / Line 1,199: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,248: / Line 1,205: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,268: / Line 1,225: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,280: / Line 1,237: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,291: / Line 1,248: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,297: / Line 1,254: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,306: / Line 1,263: @@
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:black'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt'> were extracted from the Blue gel.</span></p>
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:black'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt;color:black'> </span><span lang=EN-US style='font-size:
 .0pt'>extracted from the Blue gel was purified.</span></p>
@@ Line 1,316: / Line 1,273: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅳ</span></p>
+<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>IV</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 1,322: / Line 1,279: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 1,341: / Line 1,298: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,353: / Line 1,310: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,364: / Line 1,321: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,370: / Line 1,327: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,390: / Line 1,347: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,402: / Line 1,359: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,413: / Line 1,370: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,419: / Line 1,376: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,454: / Line 1,411: @@
 <p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>I</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Sample 1.</span></p>
@@ Line 1,467: / Line 1,424: @@
 <p class=MsoNormal><i><span lang=EN-US style='font-size:11.0pt'>ATF2</span></i><span
 lang=EN-US style='font-size:11.0pt'> gene (</span><span style='font-size:11.0pt;
-font-family:"ＭＳ 明朝","serif"'>Ⅲ</span><span lang=EN-US style='font-size:11.0pt'>)
+font-family:"ＭＳ 明朝","serif"'>III</span><span lang=EN-US style='font-size:11.0pt'>)
 extracted from the Blue gel was purified by ethanol precipitation.</span></p>
@@ Line 1,474: / Line 1,431: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
-carried out using the primers </span><span style='font-size:11.0pt;font-family:
+carried out using the primers </span></p>
-"ＭＳ 明朝","serif"'>どの？？？？</span><span lang=EN-US style='font-size:11.0pt'>.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR products were
 electrophoresed in 1% agarose gel, and several fragments were detected.</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
-carried out using the primers </span><span style='font-size:11.0pt;font-family:
-"ＭＳ 明朝","serif"'>どの？？？？</span><span lang=EN-US style='font-size:11.0pt'>.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅱ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>II</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
-carried out using the primers </span><span style='font-size:11.0pt;font-family:
+carried out using the primers </span></p>
-"ＭＳ 明朝","serif"'>どの？？？？</span><span lang=EN-US style='font-size:11.0pt'>.</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1-1 </span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3'
+TCGGCAATGCACAGTCATGGCCATAGTAGA</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>R:5'
+TTATTCAATTGGATGAGAAATCACCTTTATGAAATCATGCT</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1-2 </span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3'
+CATCTTGAATTCGATGACTTGATTATGAATAATCAACCA</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
 of <i>ATF2</i> gene were purified, and digested with <i>Mfe</i> and <i>Eco</i>R</span><span
-style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span><span lang=EN-US
+style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>I</span><span lang=EN-US
 style='font-size:11.0pt'>.</span></p>
@@ Line 1,504: / Line 1,470: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Ligation of 2-1
-and 2-2 </span><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>どれや？？？</span><span
+and 2-2 was performed using Ligation mix.</span></p>
-lang=EN-US style='font-size:11.0pt'>was performed using Ligation mix.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
-carried out using the primers </span><span style='font-size:11.0pt;font-family:
+carried out using the primers </span></p>
-"ＭＳ 明朝","serif"'>どの？？？？</span><span lang=EN-US style='font-size:11.0pt'>.(N)</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>F:3' CGATCTCTAGATGGAAGATATAGAAGGATACGAACCACAT</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>R:5' CGATCACTAGTATTAAAGCGACGCAAATTCGCCGATGGTTTG</span></p>
 <p class=MsoNormal><b><span lang=EN-US>&nbsp;</span></b></p>
@@ Line 1,517: / Line 1,485: @@
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>24th August</span></b></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>I</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 1,541: / Line 1,509: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 1,560: / Line 1,528: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,572: / Line 1,540: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,583: / Line 1,551: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,589: / Line 1,557: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,604: / Line 1,572: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif";color:black'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,621: / Line 1,589: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,632: / Line 1,600: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,638: / Line 1,606: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,651: / Line 1,619: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅱ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>II</span></p>
 <p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
@@ Line 1,673: / Line 1,641: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,690: / Line 1,658: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,701: / Line 1,669: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,707: / Line 1,675: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,727: / Line 1,695: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,739: / Line 1,707: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,750: / Line 1,718: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,756: / Line 1,724: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,777: / Line 1,745: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅲ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>III</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1<span
@@ Line 1,786: / Line 1,754: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 1,798: / Line 1,766: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif";color:black'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,815: / Line 1,783: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,826: / Line 1,794: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,832: / Line 1,800: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,845: / Line 1,813: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅳ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>IV</span></p>
 <p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
@@ Line 1,853: / Line 1,821: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 1,870: / Line 1,838: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,882: / Line 1,850: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,893: / Line 1,861: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,899: / Line 1,867: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 1,978: / Line 1,946: @@
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>29th August</span></b></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>I</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 1,991: / Line 1,959: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>２</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>II</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 2,033: / Line 2,001: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,045: / Line 2,013: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,056: / Line 2,024: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,062: / Line 2,030: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,082: / Line 2,050: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,094: / Line 2,062: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,105: / Line 2,073: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,111: / Line 2,079: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,144: / Line 2,112: @@
 <p class=MsoNormal><b><span lang=EN-US style='font-size:14.0pt'>30th August</span></b></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅰ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>I</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>1</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>PCR reaction was
@@ Line 2,166: / Line 2,134: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>R:5'</span></p>
-<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products were
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-purfied.</span></p>
+were purfied.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>It was digested
@@ Line 2,185: / Line 2,153: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,197: / Line 2,165: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,208: / Line 2,176: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,214: / Line 2,182: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,234: / Line 2,202: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,246: / Line 2,214: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,257: / Line 2,225: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,263: / Line 2,231: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,276: / Line 2,244: @@
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:windowtext'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:windowtext'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt'> extracted from the Blue gel was purified.</span></p>
@@ Line 2,289: / Line 2,257: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
@@ Line 2,301: / Line 2,269: @@
    padding:0mm 5.4pt 0mm 5.4pt'>
    <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-   style='font-size:11.0pt;color:black'>ATF2</span></span><span
+   style='font-size:11.0pt;color:black'><i>ATF2</i></span></span><span
    style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif";color:black'>　</span></p>
    </td>
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,318: / Line 2,286: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,329: / Line 2,297: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,335: / Line 2,303: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,344: / Line 2,312: @@
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>The PCR products
-of <span class=MsoSubtleEmphasis><span style='color:windowtext'>ATF2 </span></span>gene
+of <span class=MsoSubtleEmphasis><span style='color:windowtext'><i>ATF2</i> </span></span>gene
 was purified.</span></p>
 <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>&nbsp;</span></p>
-<p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>Ⅱ</span></p>
+<p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>II</span></p>
 <p class=MsoNormal><span style='font-size:11.0pt;font-family:"ＭＳ 明朝","serif"'>１</span></p>
@@ Line 2,396: / Line 2,364: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,408: / Line 2,376: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,419: / Line 2,387: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,425: / Line 2,393: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,445: / Line 2,413: @@
    <td width=56 valign=top style='width:42.25pt;border:solid windowtext 1.0pt;
    border-left:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>25&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,457: / Line 2,425: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>2&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,468: / Line 2,436: @@
    none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
    padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>3&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,474: / Line 2,442: @@
    <td width=135 colspan=2 valign=top style='width:101.2pt;border:solid windowtext 1.0pt;
    border-top:none;padding:0mm 5.4pt 0mm 5.4pt'>
-   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30uL</span></p>
+   <p class=MsoNormal><span lang=EN-US style='font-size:11.0pt'>Total 30&micro;L</span></p>
    </td>
   </tr>
@@ Line 2,487: / Line 2,455: @@
 <p class=MsoNormal><span class=MsoSubtleEmphasis><span lang=EN-US
-style='font-size:11.0pt;color:windowtext'>ATF2</span></span><span lang=EN-US
+style='font-size:11.0pt;color:windowtext'><i>ATF2</i></span></span><span lang=EN-US
 style='font-size:11.0pt'> extracted from the Blue gel was purified.</span></p>
@@ Line 2,572: / Line 2,540: @@
 <p class=MsoNormal><span lang=EN-US>&nbsp;</span></p>
-</div>
 </div>
-</html>
+</div>