Team:Newcastle

From 2013.igem.org

(Difference between revisions)
 
(147 intermediate revisions not shown)
Line 2: Line 2:
<html>
<html>
<head>
<head>
-
         <script src="http://majorwebdesigns.co.uk/iGEM_Designs/js/carousel.js" type="text/javascript"> </script>
+
         <script src="https://2013.igem.org/Team:Newcastle/script/carousel.js?action=raw&ctype=text/javascript" type="text/javascript"" type="text/javascript"> </script>
</head>
</head>
<body>
<body>
-
<!-- RSS FEED -->
 
-
<!--
 
-
<div id="rss_feed_cover"></div>
 
-
    <div id="rss_feed">
 
-
        <div id="title">
 
-
            <h2>RSS Feed</h2>
 
-
        </div>
 
-
        <div id="rss_content">
 
-
            <h4>BareCillus</h4>
 
-
            <p style="color:yellow; text-align:center;">
 
-
                Click anywhere on the Feed to lock it open!
 
-
            </p>
 
-
            <hr class="blue"/>
 
-
            <ul>
 
-
                <li>RSS item 1</li>
 
-
                <li>RSS item 2</li>
 
-
                <li>RSS item 3</li>
 
-
                <li>RSS item 4</li>
 
-
                <li>RSS item 5</li>
 
-
            </ul>
 
-
            <p>
 
-
                Paragraphs and important dates can be used to elaborate on list items. I'm padding out this paragraph as a test to see what happens to all the formatting.
 
-
            </p>
 
-
            <p>
 
-
                Let me know on thoughts guys :)
 
-
            </p>
 
-
        </div>
 
-
    </div>-->
 
-
    <!-- End of RSS Feed -->
 
     <div class="container_12 cont">
     <div class="container_12 cont">
-
<!--
 
-
<div class="clear"></div>
 
-
<div class="grid_4" id="twitter_feed" onclick="window.open('http://twitter.com/WastedGuitarist/');">
 
-
<h2>
 
-
<a href="http://twitter.com/WastedGuitarist/" target="_blank"><img src="http://www.majorwebdesigns.co.uk/iGEM_Designs/images/twitter-bird-light.png" alt="@WastedGuitarist Twitter" />@WastedGuitarist</a>
 
-
</h2>
 
-
</div>
 
-
<div class="clear"></div>-->
 
-
<div id="social_media">
 
-
<div class="box fb">
 
-
<div class="social_logo">
 
-
<img src="https://static.igem.org/mediawiki/2013/3/3a/BareCillus_fb.png" />
 
-
</div>
 
-
</div>
 
-
<div class="box twitter">
 
-
<div class="social_logo">
 
-
<a href="http://twitter.com/BareCillus" target="_blank"><img src="https://static.igem.org/mediawiki/2013/b/b1/BareCillus_twitter.png" /></a>
 
-
</div>
 
-
</div>
 
-
<div class="box youtube">
 
-
<div class="social_logo">
 
-
<img src="https://static.igem.org/mediawiki/2013/6/6a/BareCillus_Youtube.png" />
 
-
</div>
 
-
</div>
 
-
<div class="clear">&nbsp;</div>
 
-
</div>
 
<div class="clear"></div>
<div class="clear"></div>
<div id="carousel" class="grid_12">
<div id="carousel" class="grid_12">
<div id="wrapper">
<div id="wrapper">
<div class="full" id="carousel-1">
<div class="full" id="carousel-1">
-
<a href="https://2013.igem.org/Team:Newcastle/Team"><img src="https://static.igem.org/mediawiki/igem.org/e/e0/BareCillus_Meet_the_team.jpg" class="full" id="carousel-1" /></a>
+
<a href="https://2013.igem.org/Team:Newcastle/Project/L_forms"><img src="https://static.igem.org/mediawiki/2013/a/ab/Barecillus_L-.jpg" class="full" id="carousel-1" /></a>
</div>
</div>
<div class="full" id="carousel-2">
<div class="full" id="carousel-2">
-
<a href="https://2013.igem.org/Team:Newcastle/L_forms"><img src="https://static.igem.org/mediawiki/2013/a/a7/BareCillus_Carousel_FLF.jpg" class="full" id="carousel-2" /></a>
+
<a href="https://2013.igem.org/Team:Newcastle/Project"><img src="https://static.igem.org/mediawiki/2013/9/92/Barecillus_App_copy.png" class="full" id="carousel-2" /></a>
</div>
</div>
-
<div class="full" id="carousel-3"></div>
+
                                <div class="full" id="carousel-3">
-
<div class="full" id="carousel-4">
+
<a href="https://2013.igem.org/Team:Newcastle/Architecture"><img src="https://static.igem.org/mediawiki/2013/7/7c/Barecillus_Aqrch.jpg" class="full" id="carousel-3" /></a>
-
<a href="https://2013.igem.org/Team:Newcastle/Outreach/uk_meet"><img src="https://static.igem.org/mediawiki/igem.org/c/c7/BareCillus_Ysb1.jpg" class="full" id="carousel-4" /></a>
+
</div>
 +
                                <div class="full" id="carousel-4">
 +
<a href="https://2013.igem.org/Team:Newcastle/Team"><img src="https://static.igem.org/mediawiki/igem.org/e/e0/BareCillus_Meet_the_team.jpg" class="full" id="carousel-4" /></a>
 +
</div>
 +
<div class="full" id="carousel-5">
 +
<a href="http://www.youtube.com/watch?v=b0Kk6bKKOQ0" target="_blank"><img src="https://static.igem.org/mediawiki/2013/a/a0/BareCillus_Bertie_betty.jpg" class="full" id="carousel-5" /></a>
</div>
</div>
</div>
</div>
Line 83: Line 33:
<div class="clear"></div>
<div class="clear"></div>
     <div id="image_display" class="grid_12">
     <div id="image_display" class="grid_12">
-
         <div class="image_box box_1">
+
       
-
             <div class="under_text">
+
         <div class="left">
-
                 <h3>Our Project</h3>
+
       
-
                <hr/>
+
             <div class="title">
-
<p>
+
                 <a href="https://2013.igem.org/Team:Newcastle/Project">
-
Our project focuses on the creation and applications of L-forms: bacteria that grow without a cell wall. We propose L-forms as a novel chassis for synthetic biology. Our principle BioBrick switches <i>Bacillus subtilis</i> cells between rod-shape and L-form.
+
                <img src="https://static.igem.org/mediawiki/2013/b/bf/BareCillus_L-Forms_Naked_Bacteria.png" width="500"/>
-
</p>
+
                </a>
-
<p>
+
            </div>
-
We will use microfluidics to attempt genome shuffling and shape-shifting. It is easier to fuse bacteria without cell walls. Fusion will cause genetic recombination, allowing directed evolution. We will put L-forms in moulds to observe if they adopt different shapes.  
+
-
</p>
+
-
<p>
+
-
L-forms exist symbiotically in plants, which we will visualise by growing GFP labelled L-forms inside seedlings. L-forms could be engineered to supply nutrients to their host. L-forms are osmotically sensitive, giving biosecurity that they lyse if they escape from the plant.
+
-
</p>
+
-
<p>
+
-
As outreach we reflected upon our project's implications with stakeholders, created a BioGame for the public and developed a workshop for those new to modelling. Finally, we evaluated the relationship between synthetic biology and architecture.
+
-
</p>
+
 +
            <div style="margin-bottom:46px;">
 +
                <h3>
 +
                    Click below to explore!
 +
                </h3>
 +
                <hr />
 +
            </div>
-
            </div>
+
             <div class="proj_box top_left">
-
        </div>
+
-
        <div class="image_box cliches">
+
-
             <div class="cliche_1">
+
                 <div class="display_image">
                 <div class="display_image">
-
                     <img src="https://static.igem.org/mediawiki/igem.org/8/8d/BareCillus_Achievments.png" />
+
                     <img src="https://static.igem.org/mediawiki/2013/1/12/BareCillus_Switch_graphic.png" />
                 </div>
                 </div>
                 <div class="under_text">
                 <div class="under_text">
-
                    <p>Achievements</p>
+
                <a href="https://2013.igem.org/Team:Newcastle/Parts/l_form_switch">
 +
                <img src="https://static.igem.org/mediawiki/2013/7/72/L-form_Switch.png" />
 +
                </a>
                 </div>
                 </div>
             </div>
             </div>
-
             <div class="cliche_2">
+
             <div class="proj_box top_right">
                 <div class="display_image">
                 <div class="display_image">
-
                     <img src="https://static.igem.org/mediawiki/igem.org/7/71/BareCillus_Outreach.png" />
+
                     <img src="https://static.igem.org/mediawiki/2013/6/68/BareCillus_Shuffling_graphic.png" />
                 </div>
                 </div>
                 <div class="under_text">
                 <div class="under_text">
-
                    <p>Outreach</p>
+
                <a href="https://2013.igem.org/Team:Newcastle/Project/shuffling_endosymbiosis">
 +
                <img src="https://static.igem.org/mediawiki/2013/a/a4/Genome_shuffling.png" />
 +
                </a>
                 </div>
                 </div>
             </div>
             </div>
-
             <div class="cliche_3">
+
             <div class="clear"></div>
 +
            <div class="proj_box bottom_left">
                 <div class="display_image">
                 <div class="display_image">
-
                     <img src="https://static.igem.org/mediawiki/igem.org/b/b9/BareCillus_Collaboration.png" />
+
                     <img src="https://static.igem.org/mediawiki/2013/0/04/BareCillus_Plants_graphic.png" />
                 </div>
                 </div>
                 <div class="under_text">
                 <div class="under_text">
-
                    <p>Collaboration</p>
+
                <a href="https://2013.igem.org/Team:Newcastle/Project/plants">
 +
                <img src="https://static.igem.org/mediawiki/2013/b/b2/L-forms_in_plant_copy.png" />
 +
                </a>
                 </div>
                 </div>
             </div>
             </div>
-
        </div>
+
            <div class="proj_box bottom_right">
-
        <div class="image_box box_2">
+
                <div class="display_image">
-
            <div class="slice">
+
                     <img src="https://static.igem.org/mediawiki/2013/5/55/BareCillus_Shape_graphic.png" />
-
                     <img src="https://static.igem.org/mediawiki/2013/6/60/BareCillus_Switch_brick_slice.png" />
+
-
            </div>
+
-
            <div class="display_image">
+
-
                <img src="http://www.majorwebdesigns.co.uk/iGEM_Designs/images/rndm_img.jpg" alt="Random Image" />
+
-
            </div>
+
-
            <div class="under_text">
+
-
                <h3>Box 2</h3>
+
-
                <hr/>
+
-
                <p>Fusce neque urna, ornare ut mattis eget, ullamcorper eu risus. Nam euismod odio faucibus purus ultricies ut pretium turpis mollis. Nam vitae risus vitae nunc consequat pellentesque. Mauris risus urna, volutpat eget lobortis ac, auctor vitae justo. Cras venenatis auctor aliquam. Nam ornare accumsan aliquet. Ut nunc turpis, ullamcorper ut euismod a, molestie sed eros. Maecenas eget sapien nulla, et ullamcorper mauris. Aenean bibendum erat a nisi ultricies nec sodales diam vulputate.</p>
+
-
                <p>Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Ut sed purus sem, quis auctor augue. Etiam molestie dapibus libero lacinia hendrerit. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Fusce elementum malesuada facilisis. Quisque sodales viverra massa in sollicitudin. Mauris eget massa non felis dictum porta.</p>
+
-
                <div class="spacers">
+
-
                <div class="spacer_l" style="height:50px; width:65px;"></div>
+
-
                <div class="spacer_l" style="height:15px; width:110px;"></div>
+
-
                <div class="spacer_l" style="height:15px; width:135px;"></div>
+
-
                <div class="spacer_l" style="height:15px; width:155px;"></div>
+
-
                <div class="spacer_l" style="height:20px; width:170px;"></div>
+
-
                <div class="spacer_l" style="height:20px; width:180px;"></div>
+
-
                <div class="spacer_l" style="height:20px; width:185px;"></div>
+
-
                <div class="spacer_l" style="height:50px; width:200px;"></div>
+
                 </div>
                 </div>
-
                 <p>Fusce neque urna, ornare ut mattis eget, ullamcorper eu risus. Nam euismod odio faucibus purus ultricies ut pretium turpis mollis. Nam vitae risus vitae nunc consequat pellentesque. Mauris risus urna, volutpat eget lobortis ac, auctor vitae justo. Cras venenatis auctor aliquam. Nam ornare accumsan aliquet. Ut nunc turpis, ullamcorper ut euismod a, molestie sed eros. Maecenas eget sapien nulla, et ullamcorper mauris. Aenean bibendum erat a nisi ultricies nec sodales diam vulputate.</p>
+
                 <div class="under_text">
-
            </div>
+
                 <a href="https://2013.igem.org/Team:Newcastle/Project/shape_shifting">
-
        </div>
+
                 <img src="https://static.igem.org/mediawiki/2013/d/d5/Shape_Shifting.png" />
-
        <div class="image_box box_3">
+
                 </a>
-
            <div class="slice">
+
-
                 <img src="https://static.igem.org/mediawiki/2013/3/35/BareCillus_Shape_shift_slice.png" />
+
-
            </div>
+
-
            <div class="display_image">
+
-
                 <img src="https://static.igem.org/mediawiki/2013/5/57/BareCillus_Training_img.jpg" alt="Random Image" />
+
-
                 <h2>Training Week</h2>
+
-
            </div>
+
-
            <div class="under_text">
+
-
                <h3>Training Week</h3>
+
-
                <hr/>
+
-
                <div class="spacers">
+
-
                <div class="spacer_r" style="height:50px; width:65px;"></div>
+
-
                <div class="spacer_r" style="height:15px; width:110px;"></div>
+
-
                <div class="spacer_r" style="height:15px; width:135px;"></div>
+
-
                <div class="spacer_r" style="height:15px; width:155px;"></div>
+
-
                <div class="spacer_r" style="height:20px; width:170px;"></div>
+
-
                <div class="spacer_r" style="height:20px; width:180px;"></div>
+
-
                <div class="spacer_r" style="height:20px; width:185px;"></div>
+
-
                <div class="spacer_r" style="height:50px; width:200px;"></div>
+
                 </div>
                 </div>
-
                <p>The team spent the first week of iGEM learning different basic lab techniques and modelling principles. Everything was documented and written up as a "Starter's Guide" to Synthetic Biology which can be found by clicking this box.</p>
 
             </div>
             </div>
 +
 +
       
         </div>
         </div>
-
         <div class="image_box box_4">
+
          
-
            <div class="slice">
+
        <div class="left">
-
                <img src="https://static.igem.org/mediawiki/2013/c/cd/BareCillus_Plants_slice.png" />
+
              
-
             </div>
+
             <div class="project_description">
-
             <div class="display_image">
+
                 <h2>Our Project</h2>
-
                 <img src="https://static.igem.org/mediawiki/igem.org/1/11/BareCillus_Plant_img.jpg" alt="Random Image" />
+
-
            </div>
+
-
            <div class="under_text">
+
-
                <div class="spacers">
+
-
                <div class="spacer_r" style="height:50px; width:200px;"></div>
+
-
                <div class="spacer_r" style="height:20px; width:185px;"></div>
+
-
                <div class="spacer_r" style="height:20px; width:180px;"></div>
+
-
                <div class="spacer_r" style="height:20px; width:170px;"></div>
+
-
                <div class="spacer_r" style="height:15px; width:155px;"></div>
+
-
                <div class="spacer_r" style="height:15px; width:135px;"></div>
+
-
                <div class="spacer_r" style="height:15px; width:110px;"></div>
+
-
                <div class="spacer_r" style="height:50px; width:65px;"></div>
+
-
                </div>
+
-
                <h3>L-forms in Plants</h3>
+
                 <hr/>
                 <hr/>
-
                 <p>L-forms and plants can exist in a symbiotic relationship as plants provide an osmotically suitable environment. In return, L-forms can confer benefits to their host including reducing the rate of fungal infection. We plan to wash seedlings in a solution of GFP labelled L-forms, allowing the seedlings to take up the bacteria. We will then view our L-forms inside the plant using confocal microscopy.
+
                 <!--<p>
-
 
+
                Our project focuses on the creation and applications of L-forms: bacteria that grow without a cell wall. We propose L-forms as a novel chassis for synthetic biology. Our principle BioBrick switches <i>Bacillus subtilis</i> cells between rod-shape and L-form.
-
In the future, L-forms could be engineered to supply nutrients to plants, potentially increasing crop yield in low fertility soil. L-forms are osmotically sensitive, giving the ethical advantage that they lyse if they escape from their host plant into the environment.
+
                </p>
 +
                <p>
 +
                We will use microfluidics to attempt genome shuffling and shape-shifting. It is easier to fuse bacteria without cell walls. Fusion will cause genetic recombination, allowing directed evolution. We will put L-forms in moulds to observe if they adopt different shapes.  
 +
                </p>
 +
                <p>
 +
                L-forms exist symbiotically in plants, which we will visualise by growing GFP labelled L-forms inside seedlings. L-forms could be engineered to supply nutrients to their host. L-forms are osmotically sensitive, giving biosecurity that they lyse if they escape from the plant.
 +
                </p>
 +
                <p>
 +
                As outreach we reflected upon our project's implications with stakeholders, created a BioGame for the public and developed a workshop for those new to modelling. Finally, we evaluated the relationship between synthetic biology and architecture.
 +
                </p>-->
 +
<p>
 +
We have created a novel chassis for Synthetic Biology, one that is not hindered by its cell wall. These cells are called “L-Forms” or as we prefer to call them: naked bacteria. Even better, we’ve already begun putting them to good use!
 +
</p>
 +
<p>
 +
Many of the interactions between engineering and biology, required in Synthetic Biology, would be made easier without the barrier of the cell wall.  Bacteria with a cell wall are harder to get things into and out of, harder to fuse together and won’t mould into different shapes. Our main BioBrick allows us not only to remove the cell wall, but to turn it back on again at the flick of a switch, ensuring the bacterium’s dignity remains intact!
 +
</p>
 +
<p>
 +
We can fuse L-forms together and recombine their genomes - our naked bacteria undergo sexual reproduction! ;) This can be used to shuffle genomes and perform directed evolution to produce bacteria with improved phenotypes.
 +
</p>
 +
<p>
 +
L-forms have been shown to inhabit plants, we didn’t want our naked bacteria to feel left out so we put them inside plants too. They could provide natural resistance to the plant from  pathogens, and could be used to deliver  useful molecules.
 +
<p>
 +
</p>
 +
<p>
 +
Naked bacteria cannot live outside of an osmotically suitable environment; as soon as they leave a plant or the lab, they’ll burst. They essentially have an in-built kill-switch. You won’t be finding any of our naked bacteria getting dirty in soil.
</p>
</p>
             </div>
             </div>
-
        </div>
+
           
-
        <div class="image_box box_5">
+
            <div class="cliches">
-
            <div class="slice">
+
                <div class="image_box cliche_1">
-
                <img src="https://static.igem.org/mediawiki/2013/7/70/BareCillus_Genome_shuffling_slice.png" />
+
                    <div class="display_image">
-
            </div>
+
                        <img src="https://static.igem.org/mediawiki/igem.org/8/8d/BareCillus_Achievments.png" />
-
            <div class="display_image">
+
                    </div>
-
                <img src="https://static.igem.org/mediawiki/2013/0/06/BareCillus_L_Forms_IB.png" alt="Random Image" />
+
                    <div class="under_text">
-
                <h2>L-forms</h2>
+
                        <a href="https://2013.igem.org/Team:Newcastle/Notebook/achievements">
-
            </div>
+
                        <img src="https://static.igem.org/mediawiki/2013/b/b6/BareCillus_Achievements_txt.png" />
-
            <div class="under_text">
+
                    </div>
-
                <div class="spacers">
+
                </div>
-
                <div class="spacer_l" style="height:50px; width:200px;"></div>
+
                <div class="image_box cliche_2">
-
                <div class="spacer_l" style="height:20px; width:185px;"></div>
+
                    <div class="display_image">
-
                <div class="spacer_l" style="height:20px; width:180px;"></div>
+
                        <img src="https://static.igem.org/mediawiki/igem.org/7/71/BareCillus_Outreach.png" />
-
                 <div class="spacer_l" style="height:20px; width:170px;"></div>
+
                    </div>
-
                 <div class="spacer_l" style="height:15px; width:155px;"></div>
+
                    <div class="under_text">
-
                <div class="spacer_l" style="height:15px; width:135px;"></div>
+
                        <a href="https://2013.igem.org/Team:Newcastle/Outreach/Overview">
-
                <div class="spacer_l" style="height:15px; width:120px;"></div>
+
                        <img src="https://static.igem.org/mediawiki/2013/b/b9/Barecillus_Implications_txt.png" />
-
                <div class="spacer_l" style="height:15px; width:110px;"></div>
+
                    </div>
-
                <div class="spacer_l" style="height:20px; width:65px;"></div>
+
                 </div>
 +
                 <div class="image_box cliche_3">
 +
                    <div class="display_image">
 +
                        <img src="https://static.igem.org/mediawiki/igem.org/b/b9/BareCillus_Collaboration.png" />
 +
                    </div>
 +
                    <div class="under_text">
 +
                        <a href="https://2013.igem.org/Team:Newcastle/Collaborations">
 +
                        <img src="https://static.igem.org/mediawiki/2013/c/c1/BareCillus_Collab_txt.png" /><a>
 +
                    </div>
                 </div>
                 </div>
-
                <h3>L-form</h3>
 
-
                <hr/>
 
-
                <p>An L-form is a bacterium that has no cell wall. Bacterial morphology is determined by the cell wall, and so their morphology differs from the strain of bacteria from which they are derived, giving rise to a variety of cell sizes. The cell wall is important for cell division. Binary fission is a highly conserved mechanism required for proliferation of almost all cells. Due to the absence of the cell wall, L-forms are easily transformed, so we propose to use inducible L-forms of Bacillus subtilis as a novel chassis.</p>
 
-
                <p>We are working on four themes which include: Shuffling, Recombination & Endosymbiosis; Introducing and detecting L-forms in Plants; Shape-shifting; Investigating two-component systems in L-forms. Details of the individual themes can be found under &quot;Projects&quot; on the main menu</p>
 
             </div>
             </div>
         </div>
         </div>
 +
    </div>
     </div>
     </div>
</body>
</body>
</html>
</html>
 +
 +
{{Team:Newcastle/Sponsors}}

Latest revision as of 12:09, 26 October 2013

 
X
 
IGEM Home Newcastle University

Click below to explore!

Our Project

We have created a novel chassis for Synthetic Biology, one that is not hindered by its cell wall. These cells are called “L-Forms” or as we prefer to call them: naked bacteria. Even better, we’ve already begun putting them to good use!

Many of the interactions between engineering and biology, required in Synthetic Biology, would be made easier without the barrier of the cell wall. Bacteria with a cell wall are harder to get things into and out of, harder to fuse together and won’t mould into different shapes. Our main BioBrick allows us not only to remove the cell wall, but to turn it back on again at the flick of a switch, ensuring the bacterium’s dignity remains intact!

We can fuse L-forms together and recombine their genomes - our naked bacteria undergo sexual reproduction! ;) This can be used to shuffle genomes and perform directed evolution to produce bacteria with improved phenotypes.

L-forms have been shown to inhabit plants, we didn’t want our naked bacteria to feel left out so we put them inside plants too. They could provide natural resistance to the plant from pathogens, and could be used to deliver useful molecules.

Naked bacteria cannot live outside of an osmotically suitable environment; as soon as they leave a plant or the lab, they’ll burst. They essentially have an in-built kill-switch. You won’t be finding any of our naked bacteria getting dirty in soil.

Newcastle University The Centre for Bacterial Cell Biology Newcastle Biomedicine The School of Computing Science The School of Computing Science

Retrieved from "http://2013.igem.org/Team:Newcastle"