Genome shuffling

Introduction to Project

B. subtilis L-forms will be squeezed together using microfluidics in the presence of PEG (polyethylene glycol, which facilitates cellular fusion). Fusion will result in the genomes coming into close proximity. The L-forms being fused are from the same strain; therefore their genomes will be almost identical. This will allow homologous recombination, in which similar sections of DNA are ‘swapped’ between genomes, to take place. Subsequent splitting of the fusion product will give two new cells, neither of which is identical to their ‘parents’. We visualised this shuffling by fluorescently tagging our L-forms genomes using parts [http://parts.igem.org/Part:BBa_K1185001 BBa_K1185001] and [http://parts.igem.org/Part:BBa_K1185002 BBa_K1185002], which mark bacterial genomes with HBsu-sfGFP and HBsu-RFP respectively.

This technique exploits the diversity that naturally exists between members of the same species. Parallels can be drawn between this process and meiosis, and could even be viewed as making the bacteria procreate. Our new cells will be phenotypically distinct, in the same way that a child is different from either of its parents. Some of the cells produced will have enhanced function of the trait of interest. This can be assayed for, these cells isolated, and the process repeated multiple times, giving us new and improved (for the phenotype of interest) cells. In theory, different strains, or even species, of bacteria could be fused and exhibit shuffling, increasing variation further.

Purpose

Over the past eight years hundreds of iGEM teams have dreamt up exciting and innovative ways of harnessing Synthetic Biology. However only a few iGEM projects have developed into further research. Indeed, despite the thousands of hours devoted to research, the field of Synthetic Biology has not made a viable product.

Synthetic Biology is a relatively new subject, and like similar, more established areas, such as Genetic Engineering it has not yet delivered on its potential.

It would be disingenuous to downplay the important research happening around the world in these fields. However it is undeniable that a gap exists between what we want to achieve and what is currently achievable. Whilst this lack of progress can be attributed to both the complexity of biological systems involved and a public suspicious of genetic modification, there is also the problem of producing a viable, high quality yield of the desired product.

Processes in biology can be refined and improved by evolution, but in nature this occurs very slowly. We propose using L-forms to artificially evolve B. subtilis using genome shuffling. This technique increases the rate of evolution, allowing the improvement of any biological system or phenotype in a feasible timeframe.

Selective breeding has been central to the success of agriculture. We are extending these principles to microorganisms. This is potentially very powerful, as microorganisms replicate very quickly, allowing numerous rounds of selection over short time periods.

Directed evolution of bacteria and even individual genes is being increasingly explored. For example GFP, now essential to researchers, initially needed to be modified by directed evolution to produce enough fluorescence to be useful.

In this case modification was accomplished using mutagenic PCR, which mutates a single gene. It relies on the researcher knowing, and having sequence data on what genes govern the phenotype of interest. But many genetic circuits are poorly understood or controlled by many different genes, each of which could potentially have its function improved. Or it may even be desirable to engineer multiple phenotypes simultaneously. Genome shuffling is therefore a powerful tool for refining complex systems, as it effects the whole genome and requires no knowledge of how the system being engineered works.

Protoplasts, cells in which the cell wall has been removed with an enzyme such as lysozyme, have already been used in fusion and genome shuffling. In the paper [http://www.nature.com/nature/journal/v415/n6872/full/415644a.html Genome shuffling leads to rapid phenotypic improvement in bacteria], the authors claim that:

‘…two rounds of genome shuffling were sufficient to achieve results that had previously required 20 rounds of CSI. Theoretically, this is the difference between the asexual process of CSI and the hypersexual process of genome shuffling. Practically, this is the difference between 24,000 assays and approximately 1 year of effort, and roughly 1,000,000 assays and 20 years of effort.’

CSI refers to classical strain improvement (random mutagenesis and screening), currently the most popular method for the development of commercial microorganisms

We believe that using L-forms rather than protoplast in genome shuffling is even more advantageous. Firstly protoplasting gram-negative bacteria such as B. subtilis is difficult due to the complexity of the cell wall. Using L-forms circumvents this problem. Furthermore L-forms, whilst fragile, are more stable and therefore easier to work than protoplasts as they are less likely to burst due to increased membrane flexibility. Unlike protoplasts, L-forms can also grow and replicate, so can comfortably kept in a cell-wall removed state for longer periods of time. Reintegration of the cell wall after experiments are complete is also easier with L-forms.

Our Objectives

The timescale of iGEM means that it isn’t feasible to actually evolve and show improvement of a phenotype of interest. However we can show that recombination is taking place, and then directed evolution would be possible. We have constructed BioBricks that code for DNA tags. These are indiscriminate DNA binding proteins fused to GFP or RFP. The BioBrick is under the control of an IPTG inducible promoter. This allows us to produce two types of cell: those which have a green fluorescing genome, and those with a red fluorescing genome. We place one of each at each end of a microfluidics chamber, and apply pressure. The cells will be pushed together and fuse, before splitting again into two cells. If recombination has taken place each cell should have both green and red fluorescence. This is because the DNA tags remain associated to the specific DNA they are bound to as bits of DNA are ‘swapped’ between genomes.

Dual expression will not be due to our fusion protein binding to DNA during the fused cell state as the cells will be removed from an IPTG containing media the night before we fuse. This is acceptable due to the long (18-24 hour) half-life of the protein. Furthermore, the protein binds strongly to DNA, so there isn’t a significant risk of it unbinding and rebinding to another DNA strand.

Results

File:GFP.tiff

References

[http://www.nature.com/nature/journal/v415/n6872/full/415644a.html Zhang, Y. X., Perry, K., Vinci, V. A., Powell, K., Stemmer, W. P. C., & del Cardayre, S. B. (2002). Nature, 415(6872), 644–646.- Genome shuffling leads to rapid phenotypic improvement in bacteria]