Aromatics Scouts

A Comprehensive Biosensor Toolkit to Profile Aromatics in Environment

Aromatic pollutants are becoming a worldwide concern. Monitoring aromatics in environment, however, remains a substantial challenge. Noting the abundant genomic data from prokaryotes living in aromatics-rich environment, Peking iGEM applied part mining to the genetic repertoire to develop a comprehensive set of transcriptional-regulator-based biosensors for aromatics. The transcription regulators for each typical class of organic compounds were first bioinformatically determined and then promoter engineering and protein engineering were performed to tune their properties functionally. To expand the detection profiles of biosensors, enzymes in upper pathways, working as plug-ins, were coupled with existing biosensors to degrade aromatics to detectable compounds. All these sensors are capable of detect a group of aromatics, and multi-sensor assay may provide an insight of detect certain components in samples.
Additionally, for the ease of detection, we have constructed a band-pass filter to detect a specific range of concentration. Responses of biosensors equipped with band-pass filter can robustly reflect the concentration of environmental samples.
In conclusion, Peking iGEM has remarkably enriched the library of biosensors for aromatics and enabled quantitative in situ detection for environmental monitoring. These biosensors as well-characterized synthetic biological tools, we expect, will be also intriguing for metabolic engineering, such as metabolic process control.