Team:Peking

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<a href="https://2013.igem.org/Team:Peking">Home</a>
<a href="https://2013.igem.org/Team:Peking">Home</a>
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<a href="https://2013.igem.org/Team:Peking/Team">Team</a>
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<a href="">Team</a>
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<li><a href="https://2013.igem.org/Team:Peking/Team/Members">Members</a></li>
<li><a href="https://2013.igem.org/Team:Peking/Team/Members">Members</a></li>
<li><a href="https://2013.igem.org/Team:Peking/Team/Notebook">Notebook</a></li>
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<a href="https://2013.igem.org/Team:Peking/Project">Project</a>
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                                 <li><a href="https://2013.igem.org/Team:Peking/Project/AutoSensorMining">Auto Sensor Mining</a></li>
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                                 <li><a href="https://2013.igem.org/Team:Peking/Project/SensorMining">Biosensor Mining</a></li>
<li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a></li>
<li><a href="https://2013.igem.org/Team:Peking/Project/BioSensors">Biosensors</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Plug-ins</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/Project/Plugins">Adaptors</a></li>
<li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a></li>
<li><a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">Band-pass Filter</a></li>
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                                 <li><a href="https://2013.igem.org/Team:Peking/Project/Devices">Devices</a></li>
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                                <li><a href="https://2013.igem.org/Team:Peking/ModelforFinetuning">Biosensor Fine-tuning</a></li>
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<a href="https://2013.igem.org/Team:Peking/HumanPractice">Data page</a>
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<a href="">Data page</a>
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<li><a href="https://2013.igem.org/Team:Peking/DataPage/JudgingCriteria">Judging Criteria</a></li>
<li><a href="https://2013.igem.org/Team:Peking/DataPage/JudgingCriteria">Judging Criteria</a></li>
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<a href="https://2013.igem.org/Team:Peking/HumanPractice">Human Practice</a>
<a href="https://2013.igem.org/Team:Peking/HumanPractice">Human Practice</a>
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                                 <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/Questionnaire">Questionnaire</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/FactoryVisit">Factory Visit</a></li>
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                                 <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/Questionnaire">Questionnaire Survey</a></li>
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                                 <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/iGEMWorkshop">iGEM Workshop</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/FactoryVisit">Visit and Interview</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/ModeliGEM">Model iGEM</a></li>
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                                 <li><a href="https://2013.igem.org/Team:Peking/HumanPractice/ModeliGEM">Practical Analysis</a></li>
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<li><a href="https://2013.igem.org/Team:Peking/HumanPractice/iGEMWorkshop">Team Communication</a></li>
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         <a href="https://igem.org/Team_Wikis?year=2013"><img id="iGEM_logo" src="https://static.igem.org/mediawiki/igem.org/4/48/Peking_igemlogo.jpg"/></a>
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         <a href="https://2013.igem.org/Main_Page"><img id="iGEM_logo" src="https://static.igem.org/mediawiki/igem.org/4/48/Peking_igemlogo.jpg"/></a>
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<div id="ProjectTitle">
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<h1 id="ProjectName">Aromatics Scouts</h1>
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<h1 id="ProjectName">Aromatics Scouts<img id="LittleScout" src="https://static.igem.org/mediawiki/igem.org/8/8a/Peking2013_home_Telescope.png" /></h1>
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                 <h1 id="ProjectSubname">A Comprehensive Biosensor Toolkit to Profile Aromatics in Environment</h1>
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                 <h1 id="ProjectSubname">A Comprehensive Biosensor Toolkit for Profiling Aromatic Compounds in the Environment</h1>
   
   
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<p id="PreLocation">
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<B>★Presentation:</B> Room 34-101, Saturday 12:30 PM, Session 2
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      &ensp;&ensp;&ensp;&ensp;<B>★Poster:</B> Sunday, #13 (Stata)
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         <img id="MajorBodyBackground" src="https://static.igem.org/mediawiki/igem.org/9/97/Peking2013_Home_background.jpg" />
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            <p id="AbstractContent">Aromatic pollutants are becoming a worldwide concern. Monitoring aromatics in environment, however, remains a substantial challenge. Noting the power of biosensors to enable quick and convenient testing, Peking iGEM have developed a comprehensive biosensors toolkit to profile aromatics in environment. Transcriptional regulators sensing each typical class of aromatic compounds were first bioinformatically determined using the genomic data from prokaryotes, and then utilized to build biosensor circuits in living cells. Genetic tailoring such as promoter engineering was performed to tune their properties functionally. Each of these novel biosensors is proved to be capable of faithfully sensing a specific group of aromatics. Furthermore, the orthogonality/crosstalk of their detection profiles was carefully examined; this allowed the combination of these biosensors to profile aromatics for the ease of practical applications.  
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              <p id="AbstractContent">Monitoring aromatic compounds in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.  
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<br/><br/>
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Besides, to expand the detection profiles of some biosensors, enzymes of aromatics-metabolizing were gleaned from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits. Additionally, for the ease of practical analysis, we have constructed a genetic device called "Band-pass Filter" to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter proved to robustly quantify the aromatics in environmental samples.  
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Transcriptional regulators that each detect a specific class of aromatics were first bioinformatically determined; and then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and the orthogonality/crosstalk between them proved that these biosensors are very capable at profiling aromatics present in water.  
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In conclusion, Peking iGEM has remarkably enriched the repertoire of biosensors for aromatic compounds. These novel biosensors, together with the Adaptors and the Band-pass Filter, will serve as intriguing synthetic biological tools for diverse practical applications, such as process control in metabolic engineering.</p>
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Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable compounds into detectable compounds, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.  
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<br/><br/>
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We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.
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</p>
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               <h1 id="Appendix1Title">We have a date in the database</h1>
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               <h1 id="Appendix1Title"><i>Mining aromatics-sensing Biobricks <br/>from the genomic database</i></h1>
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                     <h1 class="AppendixHeadLine">Auto Sensor Mining</h1>
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                     <h1 class="AppendixHeadLine">Biosensor Mining</h1>
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                    <p>The core component of our biosensor toolkit is the transcriptional regulators that sense aromatics. For the comprehensiveness of aromatics-sensing, a data-mining process was conducted to mine transcriptional regulators for each typical class of aromatics from the database Uniprot. </p>
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                    <a href="https://2013.igem.org/Team:Peking/Project/SensorMining">LEARN MORE</a>
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               <h1 id="Appendix2Title">Light of glory shall shed<br/>upon those who see</h1>
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               <h1 id="Appendix2Title"><i>High-performance, profile-<br/>specific biosensors</i> </h1>
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                     <img id="AppendixImg2" src="https://static.igem.org/mediawiki/igem.org/1/17/Peking2013_home_appendix2.jpg" />
                     <img id="AppendixImg2" src="https://static.igem.org/mediawiki/igem.org/1/17/Peking2013_home_appendix2.jpg" />
                     <h1 class="AppendixHeadLine">Biosensors</h1>
                     <h1 class="AppendixHeadLine">Biosensors</h1>
                     <img id="Appendix2Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/b/b2/Peking2013_home_appendix2icon.png" />
                     <img id="Appendix2Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/b/b2/Peking2013_home_appendix2icon.png" />
 +
                    <p>A comprehensive set of biosensor circuits have been implemented using the aromatics-sensing transcriptional regulators. Each biosensor has a specific aromatics-sensing profile. Furthermore, the orthogonality of their sensing profiles was carefully assessed for practical applications.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/BioSensors">LEARN MORE</a>
               </div>
               </div>
         </div>
         </div>
         <div id="AppendixBox3" class="SmallBoxes">
         <div id="AppendixBox3" class="SmallBoxes">
-
               <h1 id="Appendix3Title">They will always be there<br/>waiting for our arrival</h1>
+
               <h1 id="Appendix3Title"><i>Converting the undetectable into<br/>the detectable</i></h1>
               <div id="Appendix3Cover" >
               <div id="Appendix3Cover" >
                     <img id="AppendixImg3" src="https://static.igem.org/mediawiki/igem.org/1/1b/Peking2013_home_appendix3.jpg" />
                     <img id="AppendixImg3" src="https://static.igem.org/mediawiki/igem.org/1/1b/Peking2013_home_appendix3.jpg" />
-
                     <h1 class="AppendixHeadLine">Plug-ins</h1>
+
                     <h1 class="AppendixHeadLine">Adaptors</h1>
                     <img id="Appendix3Icon"  class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/c/cd/Peking2013_home_appendix3icon.png" />
                     <img id="Appendix3Icon"  class="AppendixIcon" src="https://static.igem.org/mediawiki/igem.org/c/cd/Peking2013_home_appendix3icon.png" />
 +
                    <p> To expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were taken from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/Plugins">LEARN MORE</a>
               </div>
               </div>
         </div>
         </div>
         <div id="AppendixBox4" class="SmallBoxes">
         <div id="AppendixBox4" class="SmallBoxes">
-
               <h1 id="Appendix4Title">My heart beats only for the special you</h1>
+
               <h1 id="Appendix4Title"><i>Rapidly determining <br/> the analyte concentration</i></h1>
               <div id="Appendix4Cover" >
               <div id="Appendix4Cover" >
                     <img id="AppendixImg4" src="https://static.igem.org/mediawiki/igem.org/e/e6/Peking2013_home_appendix4.jpg" />
                     <img id="AppendixImg4" src="https://static.igem.org/mediawiki/igem.org/e/e6/Peking2013_home_appendix4.jpg" />
                     <h1 class="AppendixHeadLine">Band-pass Filter</h1>
                     <h1 class="AppendixHeadLine">Band-pass Filter</h1>
                     <img id="Appendix4Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/4/42/Peking2013_home_appendix4icon.png" />
                     <img id="Appendix4Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/4/42/Peking2013_home_appendix4icon.png" />
 +
                    <p>For the ease of practical analysis, a genetic device called "Band-pass Filter" has been constructed to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter can robustly quantify the aromatics in environmental samples. </p>
 +
                    <a href="https://2013.igem.org/Team:Peking/Project/BandpassFilter">LEARN MORE</a>
               </div>
               </div>
         </div>
         </div>
         <div id="AppendixBox5" class="SmallBoxes">
         <div id="AppendixBox5" class="SmallBoxes">
-
               <h1 id="Appendix5Title">Though soundless the beatings of wings<br/>do feel the pleasant breeze</h1>
+
               <h1 id="Appendix5Title"><i>Communications, Questionnaire survey,  <br/>Factory Visit</i> and interview</h1>
               <div id="Appendix5Cover" >
               <div id="Appendix5Cover" >
                     <img id="AppendixImg5" src="https://static.igem.org/mediawiki/igem.org/d/da/Peking2013_home_appendix5.jpg" />
                     <img id="AppendixImg5" src="https://static.igem.org/mediawiki/igem.org/d/da/Peking2013_home_appendix5.jpg" />
                     <h1 class="AppendixHeadLine">Human Practice</h1>
                     <h1 class="AppendixHeadLine">Human Practice</h1>
                     <img id="Appendix5Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/8/85/Peking2013_home_appendix5icon.png" />
                     <img id="Appendix5Icon" class="AppendixIcon"  src="https://static.igem.org/mediawiki/igem.org/8/85/Peking2013_home_appendix5icon.png" />
 +
                    <p>To obtain the information about public awareness and the situation of aromatics pollution, a survey including factory visit and questionnaires has been conducted. We also guided an iGEM HS team and held "Model iGEM" as a competition without competitiveness.</p>
 +
                    <a href="https://2013.igem.org/Team:Peking/HumanPractice">LEARN MORE</a>
               </div>
               </div>
         </div>         
         </div>         

Latest revision as of 18:10, 28 October 2013

Aromatics Scouts

A Comprehensive Biosensor Toolkit for Profiling Aromatic Compounds in the Environment

★Presentation: Room 34-101, Saturday 12:30 PM, Session 2     ★Poster: Sunday, #13 (Stata)

Monitoring aromatic compounds in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.

Transcriptional regulators that each detect a specific class of aromatics were first bioinformatically determined; and then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and the orthogonality/crosstalk between them proved that these biosensors are very capable at profiling aromatics present in water.

Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable compounds into detectable compounds, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.

We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.

Mining aromatics-sensing Biobricks
from the genomic database

Biosensor Mining

The core component of our biosensor toolkit is the transcriptional regulators that sense aromatics. For the comprehensiveness of aromatics-sensing, a data-mining process was conducted to mine transcriptional regulators for each typical class of aromatics from the database Uniprot.

LEARN MORE

High-performance, profile-
specific biosensors

Biosensors

A comprehensive set of biosensor circuits have been implemented using the aromatics-sensing transcriptional regulators. Each biosensor has a specific aromatics-sensing profile. Furthermore, the orthogonality of their sensing profiles was carefully assessed for practical applications.

LEARN MORE

Converting the undetectable into
the detectable

Adaptors

To expand the detection profiles of some biosensors, aromatics-metabolizing enzymes were taken from natural metabolic pathways, working as Adaptors to convert undetectable chemicals into detectable aromatics when coupled with biosensor circuits.

LEARN MORE

Rapidly determining
the analyte concentration

Band-pass Filter

For the ease of practical analysis, a genetic device called "Band-pass Filter" has been constructed to allow the detection of analyte concentration within a specific range. Biosensors equipped with the Band-pass Filter can robustly quantify the aromatics in environmental samples.

LEARN MORE

Communications, Questionnaire survey,
Factory Visit and interview

Human Practice

To obtain the information about public awareness and the situation of aromatics pollution, a survey including factory visit and questionnaires has been conducted. We also guided an iGEM HS team and held "Model iGEM" as a competition without competitiveness.

LEARN MORE

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