Questionnaire after the workshop

<h2> <p> We have provided a questionnaire to participant students. We got answers from 27 students (out of 41 participants). </p> </h2>

Questions about this workshop

<h2> <p> ・Did you enjoy the workshop?
・Why do you think that the workshop was fun?
・Were you able to understand contents of the experiment we made on this workshop?
・Do you want to participate in workshops like this time again? </p> <p> All students answered that they have had a good time at the workshop. Most participants answered that they were interested in biology from before. The second most was that experiment result was visible. They were fascinated seeing E.coli glow green. Other students answered that the lecture was interesting, or they could learn further than their high school classes.
Owing to teachers’ easy-to-understand lecture and many TA’s assist, most participants were able to understand what they were doing on the experiment, but sadly some said that they couldn’t. They said the lecture was too hard. However, all students answered that they want to try experiments like this again. </p> [experiment workshop questionnaire about workshop] </h2>

Questions about biotechnology

<h2> <p> ・Did this workshop caused you to have an interest in biotechnology or gene engineering?
・Do you feel the importance of biotechnological research?
・Do you feel the importance of studying biotechnology?
・Do you want to study biotechnology at your school? </p> <p> Answers showed that participants have strong interest in biotechnology. We are happy that students have got more interest in biotechnology through this event. </p> [experiment workshop questionnaire about Biotechnology] </h2>

Questions about GM food

<h2> <p> ・Did you learn more about GM food’s safety through this workshop?
・Do you still have difficulty buying GM food? </p> In Japan, it is thought unpleasant to eat GM food. Most participants have had some difficulty to GM food at least too. However, answers showed that many students have thought deeply about GM food and they were able to swept the bad image away by this workshop.
In the first place before they know synthetic biology, we think that it was a first step to dissolve the misunderstanding about biotechnology between public and like us who study biology. The workshop was successful. </p> [experiment workshop questionnaire about GM food] </h2>

Human Practice 2: Poster session at the University of Tokyo

<h2> <p> We have participated in iGEM Japan poster session, which was held at the University of Tokyo campus festival, “Gogatsu-sai” on May 19th. Many Japanese iGEM teams gathered at this event and introduced their project to visitors. Visitors were not only who is concerned to iGEM, there were many general people. Our main purpose was to introduce our projects to general people and ask them how they thought about our ideas. </p> </h2>

How we introduced our ideas to visitors

<h2> <p> We made stories to make our projects more familiar and easier to general people </p> </h2>

・Toggle Switch & Crosstalk : Oni-gokko

<h2> <p> Oni-gokko is a game Japanese kids play outside. Because every Japanese has experience of playing Oni-gokko, we thought it is a good example to explain the toggle switch system. A human E. coli, a demon E. coli, and a hero E. coli appear in this story. When the human E. coli is caught by a demon E. coli, it turns into zombie E. coli. When the zombie E. coli is rescued by a hero E. coli, it turns back to human E. coli. </p> </h2> [poster]

・Phage : Jojo

<h2> <p> We chose “Jojo’s bizarre adventure”, which is a famous comic in Japan, to introduce our Phage idea. In the comic, a hero handles his guardian angel and defeats many enemies. We made our story tracing this comic. A hero E. coli, An enemy E. coli, and a guardian angel phage appear in this story. When the hero E. coli gets signal from an enemy E. coli, the hero E. coli releases phages and leads the enemy E. coli to bacteriolysis. </p> </h2> [poster]

・Farmer : Farmer E. coli

<h2> <p> We are facing to the global food shortage problem. Researchers all over the world are now thinking how to cope with this. We are also researchers in the bud. We considered how we can solve this problem from a viewpoint of synthetic biology. We thought that if we could raise the yield to the theoretical value, it would be helpful to solve the problem. To realize this, we have to control plant’s growth according to the amount of nutrition in the soil. Therefore, we propose a system that if the amount of nutrition in the soil is enough, encourage plants to synthesize “Auxin”, which make leaves and seeds grow, and if it is not enough, encourage plants to synthesize “Cytokinin”, which make shoots grow to resorb more nutrition. Then how we tell the amount of nutrition to plants? We make “Farmer E. coli”. Farmer E. coli senses the amount of nutrition and emits two accelerating substances to encourage plants to synthesize “Auxin” or “Cytokinin” depending on the situation. If we could consign farming to E. coli, it would also be a significant cost reduction too. </p> </h2> [poster]

How we asked questions to visitors

<h2> <p> First, we explained what iGEM is and the meaning of synthetic biology, and then we introduced our ideas using posters according to visitors’ knowledge of biology. At the end we asked them for writing their opinions, suggestions, problems, questions on a label and sticking on our posters like the picture. The positive opinions were written on yellow labels, and the negative opinions were written on blue labels. </p> </h2>

Visitors’ reaction

<h2> <p> Visitors asked a lot of questions to us. When we say, “We use E. coli to realize our ideas. ”, many asked us “Isn’t it dangerous to handle E. coli? Don’t you get diseases from it? ”. We had to explain the safety of E. coli we use on biological experiments. Furthermore, we had to sweep away the bad image of E. coli and tell the importance of it. What we most cared for telling these things was to attract interest in synthetic biology. Many visitors asked us questions regardless of their age and knowledge, we spent the day with a very rewarding experience. We also got many stimulating opinions. </p> </h2> [poster session questionaire]

How we used the opinions we have got from visitors

<h2> <p> We read all of the incentive opinions, and took them into our projects. Some problems were pointed out by these opinions, so we tried to get over them. </p> </h2>

Back Ground

<h2> <p> From Human Practice1 and 2
•We confirmed that most general people have a negative image of genetic manipulation and E. coli in the first place before they know synthetic biology. However, most general people faced our ideas of synthetic biology earnestly. To incorporate plots which tell a story with our ideas is important for helping general people recognize clearly synthetic biology.
•We thought of a solution for food shortage from a viewpoint of the synthetic biology as a theme for helping general people understand easily genetic programming. </p> <p> Synthetic biology is similar to existing genetic modification technology in view that we modify microorganisms by using useful genes. However, synthetic biology is more advanced than existing genetic modification technology in view that we program relationships of using useful genes on the basis of mathematical model. We wanted to transmit the advanced point to society as well as enjoy ourselves through iGEM. </p> <p> When we start up this project, from Human Practice1 and 2, we confirmed that most general people have an unilateral negative image of genetic manipulation and E. coli by contact with various general people through a workshop and a poster session. For example, when we explained to general people that E. coli is used in an experiment for our ideas, they said to us " Is E. coli a unsafe germ? Is your body health? ". When we explained to them about genetic manipulation, they said to us " Genetic manipulation is not such a difficult operation. Easily making the individual that cannot be in the world formerly make me feel slightly terrible. ".
However even people who have negative image of genetic manipulation faced our ideas of synthetic biology earnestly. They also questioned and argued us many times. When the argument was finished, we asked them a question. They said to us with a smile "Well, I haven't known that E. coli and genetic manipulation are helpful to the development of science! Today, I'm glad to hear that interesting story about progressive synthetic biology! ".
Our ideas attracted them. We augured with them about our ideas. Through these experiences, we got clues that our project can be recognized by society. </p> <p> As stated above, the goal of our project is "We solve a general negative image of genetic manipulation and E. coli based upon synthetic biology. ", that is, "We solve a general negative image of genetic manipulation using microorganisms in biotechnology. ". To achieve the Goal, we must reflect knowledge from Human Practice1 and 2 on our project as much as possible. </p> <p> Things from Human Practice1 and 2 reflected on our project are the following three points below.
(1) Contain plots to help general people understand
(2) Contain contents that attract most people
(3) Appeal technology of synthetic biology </p> <p> By adding (1)~(3) to our project, we aimed to achieve the goal. In (1), when we selected stories, we set conditions that contents are famous all over the world and suggestive of Japan. We had many ideas. However, we finally selected "Ninja ". This is because we did not find other ideas to meet (2) and (3). There is something fascinating about ecology of ninja. By expressing ecology of ninja by E. coli, our project can attract many people. When we succeed in expressing ecology of ninja in genetic circuits, our project will help general people understand programming of genetic network in synthetic biology. </p> <p> However, even if we use project "Ninja ", programming of genetic network doesn't fit to appeal synthetic biological technology that is easily recognized by many people. Therefore, we started up another project "Farming " for making practicable. In this project, we aim to improve efficient agriculture for coming food shortage.
By making E. coli control plant hormone, regardless of uneven fertilized soil, we can improve drastically yield. Furthermore, making E. coli farmer reduce manpower. We implement low-cost and efficient agriculture. </p> <p> Project "Ninja" and project "Farming" are greatly promising. </p>

This picture shows that one of our members talked with schoolchildren. We decided to make our project which is understandable for all kinds of people.

<p> This picture shows that one of our members talked with schoolchildren. We decided to make our project which is understandable for all kinds of people. </p> </h2>

Human Practice 3: An Interviewed by Takeda-keisoku-sentan-chi foundation.

<h2> <p> This June, we (iGEM2012 tokyo tech leader Mr. Nakayama and iGEM2013 tokyo tech 7 members) were interviewed by Takeda-keisoku-sentan-chi foundation briefly. This foundation holds a symposium with various researchers on the basis of a big theme every year. Additionally, they publish a summary of the symposium. For example, in the symposium on the basis of "Fluctuation", Distant-controlling "Hayabusa" asteroid probe that returned from the universe June 2010, the origin of the universe and a system of biology are discussed from a viewpoint of "Fluctuation".
A symposium was held about regenerative medicine, experimental physics and synthetic biology on the basis of "Making" this year. In synthetic biology, the symposium proceeded with Prof. Kiga. Upon publishing a summary of this symposium, they hoped to make an article that is easy for general people who don't know synthetic biology to read. They wanted to refer to thought of us who are beginners of synthetic biology. Thereby, we were interviewed by Takeda-keisoku-sentan-shi foundation. </p>

</h2>

Q. What did you achieve through iGEM.

<h2> Mr. Nakayama <p> iGEM made me change study field. Though I was going to organic chemistry field originally, iGEM made me change the view of biology. Previously I had a image that all results of biology is not clear by the presence of fluctuation. However, I saw cell-cell communication operate genetic program with my own eyes, that is, I found out genetic system has "on" state and "off" state. I was interested in genetic programming. Though we used prokaryote in iGEM, I use eukaryote now. I’m studying cure of cancer on genetic level. </p> </h2>

Q.What is good points of your team.

<h2> Mr. Nakayama <p> Team Tokyo tech is different from others in Japan.We dare not to allot a part clearly. All member can experiment. All member shares project. All member can fill in lacked someone. </p> </h2>

Q.How do you active?

<h2> Shinya Suzuki <p> We hold meeting at 18:00 on Monday. The meeting is finished at 21:00 at the earliest. The meeting is finished before last train at the latest. Because team Tokyo tech is not club, we don't have place. However, our teachers lend us a room for a rest. </p> </h2>

Q. As beginner of synthetic biology, why do you participate in this competition?

<h2> Shinya Suzuki <p>

Though I'm a junior in college, I am different from other member. This year I enter here on the process of transfer admission. I was a technical junior college student until last year. I did graduation study last year. A junior in college cannot do study (like graduation study) here. Because I did graduation study hard for a year, It doesn't satisfy me to pass for a year from now as a just college student. I wanted to hold study mind. When I thought these, I knew iGEM.

</p> Sairi Tomatu <p> When I was a freshman, I took Prof. Kiga's biology lecture twice. I was interested in Prof. Kiga's research contents and the presence of iGEM. </p> Shi Hengyu <p>

I took part in this iGEM as a overseas student. Last year and the year before last, in this college, overseas students took part in iGEM. After my senior, as representative of overseas students in Titech, I want to challenge world students. If I live in Japan, I should be man who thinks as Japanese. This is why I want to feel teamwork with Japanese by experiencing various things with Japanese. I also wanted to achieve something what is cool in college.

</p> <p> When the summary of the symposium on the basis of "Making" is published, column about this interview is added to the book on two or more pages. We hope this book to be read by many people. We hope many people to recognize progressive synthetic biology and iGEM. </p>

</h2>

Human Practice 4: Young Takeda Awards

<h2> <p> Even if we use project "Ninja", it is difficult for general people to recognize programming of genetic network in synthetic biology. Accordingly, we decided to add improving efficiency of agriculture to our project as an approach on practical use side. This series of thinking was enough to motivate us. However, we eventually wanted our project to be practical application. Therefore, we had to brush up our project to be received by society. </p> <p> Thereby, we set two goals beyond about another project "Farming".
•We have to tell that our project is worthwhile for society.
•We have to learn defects of our project.
We decided to contribute a paper about our project "Farming" to Young Takeda Awards to achieve above two goals. </p> </h2>

Acknowledgment

<h2> To write this report, we got great information.
We really thank to
Prof. Hiroyuki Ohta
Prof. Daisuke Kiga
And, all people who participated in our human practices. </h2>