Team:UC Davis/Parts

From 2013.igem.org

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Our best characterized parts are our RiboTAL constructs, which consist of parts BBa_K1212011-BBa_K1212016. Among these parts, most of characterization was focused on the riboswitch+TAL constructs (BBa_K1212011, BBa_K1212012, BBa_K1212014, BBa_K1212015), as the other parts were simply pTet+GFP, which are already well characterized parts, with some extra base pairs added for our TAL binding sites (BBa_K1212013, BBa_K1212016). Both our pSB3K3 backbone (BBa_K1212002) and our GFP (K1212006) with their BsaI sites fixed were used in our RiboTAL constructs and functioned as expected but did not need separate characterization.  
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Our best characterized parts are our RiboTAL constructs, which consist of parts BBa_K1212011-BBa_K1212016. Among these parts, most of characterization was focused on the riboswitch+TAL constructs (BBa_K1212011, BBa_K1212012, BBa_K1212014, BBa_K1212015), as the other parts were simply pTet+GFP, which are already well characterized parts, with some extra base pairs added for our TAL binding sites (BBa_K1212013, BBa_K1212016). Both our pSB3K3 backbone (BBa_K1212002) and our GFP (BBa_K1212006) with their BsaI sites fixed were used in our RiboTAL constructs and functioned as expected but did not need separate characterization.  
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Revision as of 00:44, 28 September 2013

Parts

The following parts were assembled throughout the development of this project:
 Characterization  Name  Type  Description  Designer  Length 
 BBa_K1212002   Plasmid_Backbone   Golden Gate Compatible pSB3K3  Alex Neckelmann  2750 
 BBa_K1212006  Composite  pBAD+Golden Gate Compatible GFP  Aura Ferreiro  1052
 BBa_K1212009  Composite  pBAD+Riboswitch 2+GFP+T  Amy Soon  1086
 BBa_K1212010  Composite  pBAD+Riboswitch 1+GFP+T  Amy Soon  1090
 BBa_K1212011  Composite  pBAD+Riboswitch 2+TAL1  Amy Soon  2612
 BBa_K1212012  Composite  pBAD+Riboswitch 2+TAL8  Amy Soon  2616
 BBa_K1212013  Composite  pTet+TBS 1+RBS+GFP+T  Aura Ferreiro  1003
 BBa_K1212014  Composite  pBAD+Riboswitch 1+TAL 1  Amy Soon  2616
 BBa_K1212015  Composite  pBAD+Riboswitch 1+TAL 8  Amy Soon  2620
 BBa_K1212016  Composite  pTet+TBS 2+RBS+GFP  Amy Soon  1003

Our best characterized parts are our RiboTAL constructs, which consist of parts BBa_K1212011-BBa_K1212016. Among these parts, most of characterization was focused on the riboswitch+TAL constructs (BBa_K1212011, BBa_K1212012, BBa_K1212014, BBa_K1212015), as the other parts were simply pTet+GFP, which are already well characterized parts, with some extra base pairs added for our TAL binding sites (BBa_K1212013, BBa_K1212016). Both our pSB3K3 backbone (BBa_K1212002) and our GFP (BBa_K1212006) with their BsaI sites fixed were used in our RiboTAL constructs and functioned as expected but did not need separate characterization.

Project Background

Learn about how we combine riboswitches and TALs into robust orthogonal mechanisms for inducible repression.

Results

Check out the results of our experiments.

Human Practices

Take a look at how we designed a new database for better raw data characterization of Biobricks.

Judging Criteria

Here's the criteria that we met for this year's team.

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