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Team:UNITN-Trento/Parts
From 2013.igem.org
| ☺ ☺ ☺ | BBa_K1065000 | EFE |
| Ethylene Forming Enzyme gene (EFE) that forms ethylene starting from 2-oxoglutarate. | ||
| ☺ ☺ ☺ | BBa_K1065002 | EFE with double-terminator |
| EFE followed by the double terminator BBa_B0015. | ||
| ☺ ☺ ☺ | BBa_K1065001 | EFE arabinose-regulated |
| This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter. | ||
| ☺ ☺ ☺ | BBa_K1065100 | BSMT1 |
| This part encodes the BSMT1, it is the enzyme that methylates salycilic acid to obtain methyl salycilate. It’s the original part from the Eau de coli MIT iGEM 2006 project, resubmitted by us in pSB1C3. | ||
| ☺ ☺ ☺ | BBa_K1065103 | PchBA |
| PchA ,the enzyme catalyzing salicylate biosynthesis isochorismate, and PchB that is the isochorismate-pyruvate lyase for that form the precursor for the salycilic acid. | ||
| ☺ ☺ ☺ | BBa_K1065104 | SAM synthetase |
| This part encodes the SAM (S-AdoMet) synthetase gene, (which we friendly called "uncle SAM") . It is a methyl donor required to produce MeSA and other methylated molecules. | ||
| ☺ ☺ ☺ | BBa_K1065101 | BSMT1 arabinose-regulated |
| A methyltransferase (BSMT1) regulated by the araCpBAD inducible promoter. | ||
| ☺ ☺ ☺ | Bba_K1065105 | SAM synthetase IPTG-regulated |
| This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter. | ||
| ☺ ☺ ☺ | BBa_K1065102 | PchBA IPTG-regulated and BSMT1 arabinose-regulated |
| This is EFE followed by a double terminator (B0015) under the control of the araCpBAD inducible promoter. | ||
| ☺ ☺ ☺ | BBa_K1065106 | BSMT1, PchBA and SAM synthetase |
| An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production. | ||
| ☺ ☺ ☺ | BBa_K1065201 | pXyl+GFP |
| An improved version of the wintergreen device with BMST1, PCHBA (BBa_K1065102) with added SAM synthetase enzymes to boost wintergreen production. | ||
| ☺ ☺ ☺ | BBa_K1065106 | BSMT1, PchBA and SAM synthetase |
| A B. subtilis xylose inducible plasmid (from LMU Munich iGEM 2012) to produce GFPmut3b, regulated by a strong ribosome binding site (Bba_e0840). | ||
| ☺ ☺ ☺ | BBa_K1065204 | pXyl+EFE |
| EFE in pXyl vector, specific for B. subtilis. | ||
