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Team:USTC CHINA/Notebook/Protocols/Colony PCR
From 2013.igem.org
Colony PCR
Performed with Thermo Scientific Taq DNA Polymerase (recombinant), 5U/μl
| Volume (μl) | |
| 10×Taq Buffer | 5 |
| dNTP Mix, 2 mM each | 5 (0.2 mM of each) |
| Forward primer | 0.1-1.0 μM |
| Reverse primer | 0.1-1.0 μM |
| 25 mM MgCl2* | 1-4 mM |
| Template DNA | Picked from the colony after transformation |
| Taq DNA Polymerase | 0.25 U |
| ddH2O | To 10 |
| Total | 10 |
Retrieved from "http://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Colony_PCR"
