Team:USTC CHINA/Project/Results
From 2013.igem.org
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<p>E.coli BL21 proved that the standard transdermal locus does work with GFP.</p> | <p>E.coli BL21 proved that the standard transdermal locus does work with GFP.</p> | ||
<img src="https://static.igem.org/mediawiki/2013/8/82/2013ustc-china_genecircuit5.png" width="580" height="160"/> | <img src="https://static.igem.org/mediawiki/2013/8/82/2013ustc-china_genecircuit5.png" width="580" height="160"/> | ||
- | <p>Using GFP to prove the validity of a newly designed circuit is a classical way to verify the expressing of this circuit. As expressions in E.coli involve neither secretory nor sequential problems, we hoped to verify the practicality of our locus by the expression of TD1-GFP. Thus we selected pET22b,which is a common recombinant vector for plasmid construction, as our recombinant vector and E.coli BL21 as engineered bacteria . We fused sequence TD1-GFP with T7 promoter from pET22b downstream and succeeded in expressing fusion protein TD1-GFP | + | <p>Using GFP to prove the validity of a newly designed circuit is a classical way to verify the expressing of this circuit. As expressions in E.coli involve neither secretory nor sequential problems, we hoped to verify the practicality of our locus by the expression of TD1-GFP. Thus we selected pET22b, which is a common recombinant vector for plasmid construction, as our recombinant vector and E.coli BL21 as engineered bacteria . We fused sequence TD1-GFP with T7 promoter from pET22b downstream and succeeded in expressing fusion protein TD1-GFP. </p> |
<img src="https://static.igem.org/mediawiki/2013/d/d7/2013ustc-chinajiaotuTD1-GFP.jpg"> | <img src="https://static.igem.org/mediawiki/2013/d/d7/2013ustc-chinajiaotuTD1-GFP.jpg"> | ||
<div class="atfigure" align="center">Fig1. SDS PAGE shows the molecule weight of TD1-GFP</div> | <div class="atfigure" align="center">Fig1. SDS PAGE shows the molecule weight of TD1-GFP</div> | ||
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<div class="bassic-bar"> | <div class="bassic-bar"> | ||
<h3>4. Transdermal experiment</h3> | <h3>4. Transdermal experiment</h3> | ||
- | <p>TD1-HBsAg is able to penetrate the skin and keep its antigenicity </p> | + | <p>TD1-HBsAg is able to penetrate the skin and keep its antigenicity: </p> |
<br> | <br> | ||
<div style="float:left;width:106px;height:170px;padding:5px"> | <div style="float:left;width:106px;height:170px;padding:5px"> |
Revision as of 04:28, 27 September 2013