Team:Yale/Project Export

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(Introduce type 1 secretion system to export and extract PLA)
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**We found the paper by Linton 2010 where she focused on exporting PHA from engineered ''E. coli''
**We found the paper by Linton 2010 where she focused on exporting PHA from engineered ''E. coli''
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*She used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag.  This allowed the cells to export the PLA since the hlyA tag was attached to the granule
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*She used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag.   
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**This allowed the cells to export the PLA since the hlyA tag was attached to the granule
<center>[[File:PHA_export_system.jpg|400px]]</center>
<center>[[File:PHA_export_system.jpg|400px]]</center>
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Revision as of 20:43, 31 August 2013

Project Overview Validate PLA synthesis Develop bioassay Apply MAGE Introduce export system Make a bioplastic


Aims for the Project

  1. Engineer strains of E. coli to validate PLA synthesis
  2. Develop bioassay to screen PLA production
  3. Apply MAGE to optimize PLA production, guided by FBA
  4. Introduce type 1 secretion system to export and extract PLA
  5. Make a bioplastic


Introduce type 1 secretion system to export and extract PLA

  • We needed a way to export the PLA once it was synthesized by the E. coli
    • We found the paper by Linton 2010 where she focused on exporting PHA from engineered E. coli


  • She used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag.
    • This allowed the cells to export the PLA since the hlyA tag was attached to the granule
PHA export system.jpg



  • Due to the similarity between PHA granules and PLA granules we hypothesized that this same export system would allow us to export PLA from our cells.
PLAandPHAexport.png