Team:Yale/Project Export


Introduce type 1 secretion system to export and extract PLA

  • We needed a way to export the PLA once it was synthesized by the E. coli
    • We found the paper by Linton 2010 where she focused on exporting PHA from engineered E. coli

  • Ms. Linton used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag.
    • This allowed the cells to export the PLA since the hlyA tag was attached to the granule
PHA export system.jpg

  • Due to the similarity between PHA granules and PLA granules we hypothesized that this same export system would allow us to export PLA from our cells.