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From 2013.igem.org

Introduce type 1 secretion system to export and extract PLA

• We needed a way to export the PLA once it was synthesized by the E. coli
  • We found the paper by Linton 2010 where she focused on exporting PHA from engineered E. coli

• Ms. Linton used Phasin, a PHA granule associated protein that plays a role in granule formation, with a hlyA tag.
  • This allowed the cells to export the PLA since the hlyA tag was attached to the granule

• Due to the similarity between PHA granules and PLA granules we hypothesized that this same export system would allow us to export PLA from our cells.