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07/08/13
From 2013.igem.org
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Dillution of Herring sperm DNA
- Dilutions were done in 3 sets:
- Samples 1-3 were diluted in 90ul of water, where 10ul of stock DNA was added from an overnight dilution of 100um of Herring sperm DNA in 900ul of water.
- Samples 1s-3s were also diluted in 90ul of water, except the first 10um of DNA was taken from an undiluted stock Herring sperm DNA.
- Samples 1 5/45- 3 5/45 were from a known concentration of DNA stock, where 5ul of DNA was added to 45ul of water.
| Sample | Concentration (ng/ul) | 260/280 | 260/230 |
| 1kb | 534 | 1.82 | 1.95 |
| 1 5/45 | 52.7 | 1.77 | 2.05 |
| 2 5/45 | 4.6 | 1.46 | 1.88 |
| 3 5/45 | 0.1 | 0.24 | -0.29 |
| Overnight* | |||
| 1in 10 | 24.8 | 1.8 | 2.42 |
| 1 | 2.1 | 1.67 | 22.44 |
| 2 | 0.8 | 2.87 | -3.99 |
| Fresh stock | |||
| 1s | 5341.8 | 1.78 | 2.15 |
| 2s | 440.0 | 1.80 | 2.23 |
| 3s | 45.1 | 1.83 | 2.26 |
- concentration of DNA was measured using nanodrop.
*Dilutions left on the bench overnight.
Gel electrophoresis of Dilluted Herring sperm DNA
Lanes are: marker, 1 5/45, 2 5/45, 3 5/45, 1, 2, 3, 1s, 2s, 3s, lambda NT, lambda
Results are as expected. Lanes show that samples 1, 2 and 3 were degraded overnight, suggesting nuclease activity. Samples 1s, 2s and 3s are the fresh Herring sperm stock and show no degradation.
