14 August 2013

From 2013.igem.org

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Dilutions of Herring Sperm DNA

  • Add 9ul of TE buffer to 6 epindorph tubes
  • Pipette 1ul of each sonicated DNA sample 1-6 into each buffer for a 10x dilution
  • vortex thoroughly and centrifuge briefly

Running dilutions on 0.8% gel

  • 1ul of dilution was put in 14ul of water and 5ul of orange G, to make up a total volume of 20ul for each sample.
  • 5ul of 1kb ladder was used on both ends as a marker

Igem SON dna 140813.jpg

Testing for limonene

Took sample plates to chemistry lab