From 2013.igem.org

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Count colonies on agar plates

Viability controlCovered
Negative control1 contaminant
20ul of 50pg/ul135
20ul of 10pg/ul96
200ul of 50pg/ul~1600
200ul of 10pg/ul944

Therefore there was 1.2x10^8 cfu/ug

Replate to get individual colonies

  • Take an individual colonies
  • Streak out onto new plate
  • Sterilize loop using bunsen burner
  • Incubate at 37 overnight