From 2013.igem.org

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Incubate in broth

  • 4ml of luria broth in 6 universals
  • Add 8ul of chloramphenicol
  • Pick single colony and put in broth (sterile technique)
  • Incubate overnight at 37 in shaker

Make agarose gel

  • 20ml of 5xTBE
  • 80ml of water
  • 0.8g of agarose

(0.8% gel)

  • boil till all agarose dissolved
  • Cool
  • Add 5ul of ethidium bromide
  • Pour gel

Make buffer

  • 50ml of 5xTBE
  • 200ml of water