From 2013.igem.org

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Running the gel from sonication series

  • Samples were taken from sonicated tube every 30min intervals, a total of 6 samples.
  • Each was diluted 100 fold to get a concentration in the range of 40-50ng/ul
  • 5ul of DNA was taken from the dilution, to which 10ul of sterile water and 5ul of orange G was added
  • Samples were run against a 1kb ladder and control herring sperm DNA, which was not sonicated.