From 2013.igem.org

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Isolating RFP plasmid

  • Add 1.5ml of overnight culture to epindorph
  • Centrifuge for 2mins
  • Remove supernatent
  • Repeat
  • Use thermo scientific mini kit
  • Measure concentration using nanodrop

Double digest

  • 50ng of DNA
  • 2ul of 3.1 buffer
  • 1ul of each restriction (XbaI and PstIin the 6 samples, EcoRI and PstI in the 6 samples, 12 samples in total)
  • Water to make total volume to 20ul
  • Incubate at 60mins at 37
  • 20mins at 80