From 2013.igem.org

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Making and running an agarose gel

  • Making a 3% agarose gel to run Tod PCR fusion genes from 17/09
  • This is to determine that the digestion works
  • Running the gel with digested and undigested samples to observe the difference
  • Digested samples were taken from the digestions the Tod genes from previous day
  • 100ng of both samples were run against a 100kb ladder

Morninggel 180913.jpg

Rows are as follow:100bp ladder, undigested x, digested x, undigested b, digested b, undigested f, digested f

Plating out TOD operon bacteria

  • For selection of individual colonies