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18/09/13
From 2013.igem.org
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Making and running an agarose gel
- Making a 3% agarose gel to run Tod PCR fusion genes from 17/09
- This is to determine that the digestion works
- Running the gel with digested and undigested samples to observe the difference
- Digested samples were taken from the digestions the Tod genes from previous day
- 100ng of both samples were run against a 100kb ladder
Rows are as follow:100bp ladder, undigested x, digested x, undigested b, digested b, undigested f, digested f
Plating out TOD operon bacteria
- For selection of individual colonies
