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From 2013.igem.org

Miniprep from overnight broths

• DNA is isolated using Omega Miniprep kit and its protocol is followed

Nanodropping the isolated DNA

Sample	Volume ul	Concentration ng/ul	260/280	260/230
X12	90	13.5	1.86	1.38
X3	43	13.6	2.12	1.34
X5	45	16	2.08	1.64
X6	44	19.1	2.04	1.59
B4	93	11.1	1.73	1.28
B5	87	9.5	1.99	1.34
B9	43	13.1	2.14	1.43
B12	43	14.9	1.99	1.23
F1	92	17.6	1.91	1.15
F8	93	17.6	1.85	1.70
F10	91	12.4	1.99	1.30
F12	41	18.5	2.06	1.71

Double digest of isolated DNA

• Digesting with EcorI-HF/PstI-HF and XbaI/PstI to see if DNA incorporated insert has the biobrick prefix and suffix present.
• Gel to be run in the 23/09/13