Exeter/10 July 2013

Morning

- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122] (cyan pigment) comes with the machinery to produce our pigment, albeit in low concentrations. 1 ml of the BBa_K322122 liquid cultures from yesterday were transferred to eppendorf tubes and span down into a pellet at 13000 rpm for 5 minutes. All pellets were white, meaning further investigation is required to produce a colour.

MiniPrep of the following Parts:

- [http://parts.igem.org/Part:BBa_K322122 BBa_K322122], cyan pigment

- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment

- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment

Plasmids extracted are now in storage at -20°C.

Afternoon

Liquid cultures of the blue light module and magenta pigment

- [http://parts.igem.org/Part:BBa_K592010 BBa_K592010], yellow pigment

- [http://parts.igem.org/Part:BBa_K592012 BBa_K592012], magenta pigment

- [http://parts.igem.org/Part:BBa_B0015 BBa_B0015], a terminator

- [http://parts.igem.org/Part:BBa_K592005 BBa_K592005], FixJ protein intermediate

- [http://parts.igem.org/Part:BBa_K592004 BBa_K592004], blue light sensor

Retry of BBa_B0034 transformation

Normal transformation protocol from 4/7/13 followed. Made certain we were using the right antibiotic plates!

(Next day, results of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034] transformation)

We made two plates of [http://parts.igem.org/Part:BBa_B0034 BBa_B0034], each plate had 7 colonies. Our RFP control had 137 colonies.

Take me back to the notebook.