Exeter/7 August 2013

Glycerol stocks

• 200ul of glycerol and 200ul of our overnight liquid cultures into labelled eppendorfs.
• Store at -80 degrees celsius
• Stocks made of: NEB1, Promotor + RBS, NEB4, Cyan, RBS+Cph8, Magenta, B0015, B0034, RFP control, NEB3, Yellow

We miniprepped:

• [http://parts.igem.org/Part:BBa_B0034?title=Part:BBa_B0034 B0034] (RBS) AMP

• [http://parts.igem.org/Part:BBa_K592010 K592010] (yellow) CAM

• [http://parts.igem.org/Part:BBa_S05058 S05058] (RBS + yellow) CAM

• [http://parts.igem.org/Part:BBa_K608002?title=Part:BBa_K608002 K608002] (Promoter and RBS) CAM

• [http://parts.igem.org/Part:BBa_J04450 RFP control] CAM

• 3 NEB (RFP and Terminator) AMP

• [http://parts.igem.org/Part:BBa_K592012 K592012] (Magenta) CAM

• NEB4 (RBS and cph8) AMP

• NEB1 (RBS and cph8) AMP

• [http://parts.igem.org/Part:BBa_B0015 B0015] (Terminator) CAM

• [http://parts.igem.org/Part:BBa_K592018 K592018] (RBS + Cyan) CAM

Adam and Tom

Digested ligations from Tuesday out of plasmid and ran on a gel ( but used the wrong ladder! Hyper Ladder 2 and ran off the end of the gel)

Take me back to the notebook.