- Phage Purification
- March-April
- May-June
- July-August
- September-October
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9.9 10X Phage Suspension Buffer Creation
I) Purpose
- Create a 10X solution of Phage Suspension Buffer that will be used to run our Phage Purification Centrifugation.
II) Expected Outcome
- We should have a working phage suspension buffer that will facilitate movement of phage in the CsCl gradient.
III) Reagants Used
- Tris-HCl
- MgCl2
- NaCl
- Ultrapure H20
- NaOH
IV) Actual Procedure
- Add 58.44 g of NaCl to a 1 Liter Flask to achieve a 1 M solution.
- Add 20.33 g of MgCl2 to the same 1 Liter Flask to achieve a .1 M solution.
- Add 100 mL of 1 M concentration of tris-HCl 6.8 ph to the same flask.
- Fill the flask with ultrapure H2O until it reaches the 1 L mark.
- With a pH reader read the pH of the solution.
- Add 5 microliters of NaOH at a time until the solution reads 7.2-7.5 pH on the pH meter.
V) Results
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