Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer

From 2013.igem.org


Phage Purification September - August Notebook: Experiments



Phage Purification
March-April
May-June
July-August
September-October

9.9 10X Phage Suspension Buffer Creation


I) Purpose

Create a 10X solution of Phage Suspension Buffer that will be used to run our Phage Purification Centrifugation.

II) Expected Outcome

We should have a working phage suspension buffer that will facilitate movement of phage in the CsCl gradient.

III) Reagants Used

Tris-HCl
MgCl2
NaCl
Ultrapure H20
NaOH


IV) Actual Procedure

Add 58.44 g of NaCl to a 1 Liter Flask to achieve a 1 M solution.
Add 20.33 g of MgCl2 to the same 1 Liter Flask to achieve a .1 M solution.
Add 100 mL of 1 M concentration of tris-HCl 6.8 ph to the same flask.
Fill the flask with ultrapure H2O until it reaches the 1 L mark.
With a pH reader read the pH of the solution.
Add 5 microliters of NaOH at a time until the solution reads 7.2-7.5 pH on the pH meter.

V) Results