Team:BYU Provo/Notebook/Phage Purification/Summerexp/Period1/Exp/7.5 Bacterial Preparation


Phage Purification July - August Notebook: Experiments

Phage Purification

7.5 Bacterial Preparation

I) Purpose

Grow bacteria for later use in propagation of phage.

II) Expected Outcome

Viable colonies of E. coli, both E. coli B and BL21 that can be stored in the fridge for one week.

III) Reagants Used

E. coli B and BL21 colonies from plates
LB plates

IV) Actual Procedure

Streak colony of bacteria in a quadrant, standard streak pattern and leave in shaker at 37 overnight. Parafilm and move to fridge.

V) Results

Growth of both bacterial types with isolated colonies available. The bacteria will be stored and viable for one week.