|
- Phage Purification
- March-April
- May-June
- July-August
- September-October
|
5.5 Bacteria Cultures, 25 mL
I) Purpose
- Grow bacteria for later use in purification of phage.
II) Expected Outcome
- Viable liquid cultures of E. coli, both W3110 and BL21
III) Reagants Used
- W3110 and BL21 freezer stock
- LB
IV) Actual Procedure
- Infect 25 mL of LB with 1 loop-full of bacterial freezer stock and leave in shaker at 37◦ overnight.
V) Results
- Growth of BL21 liquid cultures of bacteria to infect and amplify amounts of phage. W3110 failed to grow, so the experiment will have to be repeated.
|
"
