Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/5.6 Phage From Lysate

Phage Purification May - June Notebook: Experiments

Phage Purification

March-April

5.6 Phage From Lysate

I) Purpose

Separate Phage from bacterial lysate to store viable phage for extended periods of time.

II) Expected Outcome

Viable phage, easily stored.

III) Materials Used

2 mL eppendorf tubes (Qty 20)

5 mL cultures of BL 21 (infected with T7 phage for 24 hours) and W3110 (infected with T4 phage for 7 hours)

IV) Actual Procedure

Separated the 5 mL cultures into 5 eppendorfs, 1 mL in each.

Centrifuged at 3050 rpm for 5 min

Gently extracted the supernatant and placed into clean eppendorf tubes.

V) Results

The phage were still viable and are stable when stored in the fridge.