Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/6.7 Phage Suspension Buffer Preparation

Phage Purification May - June Notebook: Experiments

Phage Purification

March-April

6.7 Phage Suspension Buffer Purification

I) Purpose

Create 1 L of 10X Phage Supension Buffer stock.

II) Expected Outcome

Clean, usable stock of phage suspension buffer.

III) Reagants Used

NaCl

MgCl2

Tris-HCl

IV) Actual Procedure

Put 12.4 g Tris-HCl in a 1 L flask. Added 1 L H2O. Titrated to 7.2 pH using 50% NaOH. (.1 M Tris-HCl)

Added 58.44 g NaCl (1M)

Added 9.52 g MgCl2 (.1M)

Autoclaved and stored in a sealed bottle for use.

V) Results

Created 1 L of 10X phage suspension buffer