From 2013.igem.org

19 September 2013

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Lab 115

Main purpose

The purpose of this experiment is to measure nitric and nitrous oxide from the Nir transformant anaerobically while we spike it with nitrite.

The Experiment 2 describes further our investigation.

Who was in the lab

Kasia, Ariadni

Procedure

This experiment is based on the Protocol of Experiment 2 for the Nir transformant, with some changes.

The experiment started with a temperature of around 37 _o C, initial OD of 0.1036 and initial volume of 200 ml. After resuspension the OD was really low around 0.0329.

The experiment starts, taking one sample and after 7 minutes, a second spike and then we spike with 2ml of nitrite and we take two samples. Afterwards we also spike with 2 ml of nitrite again and finally take one more sample.

Results

Colorimetric results

Ranges

Measuring range 5-150 mg/L NH₄-N
Measuring range 0.02-1 mg/L NO₂-N

salt (mg/L)	sample 1	sample 2	sample after spike	sample 4	sample 5
ammonium	<2	<2	<2	<2	<2
nitrite	<0.02	<0.02	0.74	1.24	1.34

Nir Transformed E. coli

Conclusion

The NO sensor was drifting and no proper results were obtained.