208 lab

Main purposes today

Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.

Who was in the lab

Kristian, Helen

Procedure

Followed Openwetware procedure for periplasmic extraction(link). Used smaller amount though.

Then, viewed the periplasmic and cytoplasmic cell contents under UV light.

Conclusion from today

The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.

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