208 lab

Main purposes today

Extract periplasmic contents from TAT21, SEC2, TAT31a, TAT22 transformants.

Who was in the lab

Kristian, Helen

Procedure

Followed Openwetware procedure for periplasmic extraction([http://drummond.openwetware.org/Periplasmic_Prep.html link]). Used smaller amount though.

Then, viewed the periplasmic and cytoplasmic cell contents under UV light.

Conclusion from today

The Hello World project was a success -- the periplasm contains GFP and the cytoplasm contains RFP.

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