208

Main purpose

Make colony PCR on Nitrosomonas Europaea to extract the three AMO subunits. Test which volume of liquid culture is the best.

Who was in the lab

Kristian

Procedure

• AMO1 = 1uL liquid culture
• AMO10 = 10uL liquid culture
• AMO20 = 20uL liquid culture

All reactions where made in triplicates 50uL reactions and mastermix was made as described in methods just with less MQ if template volume was over 1uL.

All tubes were run on standard PCR-program with 56°C annealing temp. and 2:00 extension time.

Results

See tomorrows gel.

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