Team:EPF Lausanne/Calendar/10 August 2013

From 2013.igem.org

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Moving on
-Miniprep of cells transformed with INP_Strep-Dead gibson product.
-DpnI digestion of Streptavidin iGEM (BBa_K283010) PCR product.
-Gibson assembly of INP amplified construct and streptavidin iGEM PCR purified product.
-Plating of Streptavdin alive received from Oxford University.


Sensing-Effector

Making a glycerol Stock
-We made a 50% glycerol stock of the Bacteria that we inoculated the day before.