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8hrs inoculation at 37°C, in LB+Chloramphenicol of a colony from the INP_Strep-Dead transformation plate (06.08.13).
Additional 8hrs incubation at 37°C of the samples to make a pellet for Miniprep.
Sensing-Effector
Inoculating Bacteria
We inoculated the bacteria transformed the day before in order to do a Miniprep according to the manufacturers instructions of the qiagen Kit.