Team:HokkaidoU Japan/Notebook/RBS SD2,4,6-Reconstruction

From 2013.igem.org

HOKKAIDO U

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• Maestro E. coli
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Maestro E.coli

Notebook

SD2,4,6 Reconstruction

• Start

• PCR

  • Sat Jun 15

  R0040-SD8-LacZα-B0015 100up SD(6/4/2) mutation revers

• Gel extraction

  • Sat Jun 15

  R0040-SD(6/4/2)

• Digestion

  • Sat Jun 15

  Cut R0040-SD(6/4/2) with EcoRI and SpeI (10×H buffer)

• Ethanol precipitation

  • Sun Jun 16

  R0040-SD(6/4/2)

• Ligation

  • Sun Jun 16

  Ligate R0040-SD(6/4/2) with LacZα-B0015

• Transformation

  • Mon Jun 17

  R0040-SD(6/4/2)-LacZα-B0015
  each of 5µL DNA to DH5α

• Sequencing

  • Sat Jun 22

  R0040-SD(6/4/2)-LacZα-B0015

• Complete!

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