Team:KAIT Japan/Notebook


KAIT Japan 2013RogoTop.png

KAIT Japan2013 Kanako.png KAIT Japan 2013 iGEMRogo.png

KAIT Japan home.png Home
KAIT Japan team.png Team
KAIT Japan project.png Project
KAIT Japan parts.png Parts
KAIT Japan protocol.png Protocol

KAIT_Japan_notebook.png Notebook

KAIT Japan results.png Results
KAIT Japan safety.png Safety
KAIT Japan human practice.png Human Practice


We made an experiment every day. It was process at trial and error.

Basic task

Date:8/19 Miniprep
Date:8/20 Miniprep
Date:8/22 Miniprep
Date:8/28 Miniprep
Date:9/3 Miniprep
Date:9/12 Miniprep

Creating parts of HlyA and pGLO

Date:8/8 Design of the primer
Date:8/9 Transformation
Date:8/12 Subcluture
Date:8/13 Colony PCR of HlyA and Electrophoresis
Date:8/16 Transformation,Subcluture and Electrophoresis
Date:8/22 PCR of HlyA
Date:8/27 PCR and Electrophoresis
Date:9/11 The refinement of DNA
Date:9/11 Infusion

Creating parts of GFP

Date:8/20 Colony PCR and Electrophoresis
Date:8/21 Colony PCR and Electrophoresis
Date:8/23 PCR and Electrophoresis
Date:8/24 PCR and Electrophoresis
Date:8/28 PCR
Date:8/29 PCR and Electrophoresis
Date:8/30 Subcluture and PCR
Date:9/5 Subcluture
Date:9/6 Colony PCR and Electrophoresis
Date:9/9 PCR
Date:9/10 PCR
Date:9/18 PCR and Transformation
Date:9/19 Colony PCR

Creating parts of IL-10AR,IL-10BR and STAT3

Date:8/30 Colony PCR and Electrophoresis
Date:8/31 Colony PCR and Electrophoresis
Date:9/5 Colony PCR and Electrophoresis of IL-10AR and IL-10BR
Date:9/6 Colony PCR and Electrophoresis of STAT3
Date:9/6 PCR and infusion of IL-10AR and IL-10BR
Date:9/9 Infusion of IL-10R
Date:9/9 PCR of STAT3
Date:9/11 Infusion of IL-10R and STAT3