Team:Paris Bettencourt/Notebook/Trojan Horse/Friday 23rd August.html

Trojan Horse

23^th August

Results: failure: as much GFP+ colonies growing on Kan than on LB

PCR from mini Gene sRNA anti Cm

35 H2O

10 Buffer 5x

1 dNTP

2 FW iT009

2 RV iT0010

0.2 minigene-plasmid amp from IDT

0.5 phusion

PCR Phusion Gradient around 53 °C

Time : 30 secondes