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Team:Paris Bettencourt/Notebook/Trojan Horse/Friday 28th June.html
From 2013.igem.org
Trojan Horse
ASDF28th July
sRNA design
red sequences:
pACYCDuet-1 (CmR) : ATATCCAGTGATTTTTTTCTCCAT
pCOLADuet-1 (KanR) : CGTTTCCCGTTGAATATGGCTCAT
We decided that targeting only two different antibiotic resistance will be enough for a proof of concept. We give up AmpR because otherwise they might be experimental issues with AmpR that is also on phagemid litmus28i.
