# Team:Paris Bettencourt/Notebook/Week4

### From 2013.igem.org

## Contents |

## Week 4

### Monday 24^{th} June

### Tuesday 25^{th} June

### Wednesday 26^{th} June

### Thursday 27^{th} June

### Friday 28^{th} June

#### Trojan Horse

sRNA design

Red sequences:

pACYCDuet-1 (CmR) : ATATCCAGTGATTTTTTTCTCCAT

pCOLADuet-1 (KanR) : CGTTTCCCGTTGAATATGGCTCAT

We decided that targeting only two different antibiotic resistance will be enough for a proof of concept. We give up AmpR because otherwise they might be experimental issues with AmpR that is also on phagemid litmus28i.