Team:SydneyUni Australia/Project


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Brief Project Description


  • 1,2-dichloroethane (DCA) is part of a family of chlorinated hydrocarbons that are primarily derived from industrial solvents such as tetrachloroethene (PCE) and trichloroethene (TCE). Some of these organochlorines are toxic, carcinogenic and generally nasty.
  • DCA is a soluble and mobile contaminant of the groundwater in Botany Bay, Sydney, but also elsewhere around the world.
  • Conventional treatment involves significant costs, such as the pumping and heat-stripping of groundwater at Botany Bay. A biological alternative may be cheaper and more effective.
  • A suite of techniques (including sampling of contaminated sites, growth in bioreactors with selective conditions and protein engineering) have led to an understanding that there are two primary pathways of DCA-degradation (Fig. 1).


  • Construct a BioBrick-compatible vector inspired by the broad host range vector, pBBR1MCS2.
  • Construct and compare two of the proposed pathways of DCA biodegradation.
  • Characterise the components of the DCA-degradation pathway for admission into the Registry of Standard Parts.
  • (If we get time!) Demonstrate the integration of our the pathway in the chromosome of Pseudomonas stutzeri via natural transformation and site-specific recombination.