Team:TU Darmstadt/materials/Buffers

From 2013.igem.org







Lab book | Materials | Protocols

Buffers


Tris (1M, pH 7,5)

  • 60,5g Tris base
  • adjust pH to 7,5 using 5M HCL
  • add to 500 mL>

1x PBS

  • 8.18 g NaCl (140 mM)
  • 0.2 g KCl (2.7 mM)
  • 1.77 g Na2HPO4 (10 mM)
  • 0.24 g KH2PO4 (1.8 mM)
  • Add. 1L dH2O
  • Adjust to pH 7.4

TE buffer

  • 10 mM Tris, bring to pH 8.0 with HCl
  • 1 mM EDTA (Ethylenediaminetetraacetic acid)

50x TAE

  • 242g Tris base in water
  • add 57.1mL glacial acetic acid
  • 100mL of 500mM EDTA (pH 8.0) solution
  • add up to 1 litre of water