Team:TU Darmstadt/materials/Primers

From 2013.igem.org







Lab book | Materials | Protocols

Primers

VR: ATTACCGCCTTTGAGTGAGC
VF2: TGCCACCTGACGTCTAAGAA

Suffix-R: TACTAGTAGCGGCCGCTGCAGGGTAA
Prefix-R: GTAGTAGAATTCGCGGCCGCTTCTAG

LO-suf-R: GTAGTACTGCAGCGGCCGCTACTAGTATTATGATTTGTACAGCTCGTCCATGCC
LO-pre-F: GTTGTTGAATTCGCGGCCGCTTCTAGATGGTGAGCAAAGGCGAAGAAAATAACATGG

mKate-suf-R: GTAGTACTGCAGCGGCCGCTACTAGTATTATTAATTCAGTTTATGACCCAGGTTGCTCG
mKate-pre-ATG-F: GTTGTTGAATTCGCGGCCGCTTCTAGATGGTAAGCAAAGGTGAAGAGTTAATCAAGGAGA

lssmorange ppr: CCCCCTGCAGGTTTATTATTTGTACAGCTCGTCC
lssmorange pprf: ATATGGCTAGCATGGTGAGCAAAGGCG

mkate ppr: GTCCCCCTGCAGGTTTATTAATTCAGTTTATG
mkate pprf: TACATATGGCTAGCATGGTAAGCAAAGGTG

frag4_rev: CTCTTTCCGCTAGCCCAAAAAAACGGTATGGAG
frag1_for: CGGCCGCTTCTAGAGACATTGATTATTTGCACGGCGT

frag2_rev: CAGTGTGATTATTAATTCAGTTTATGACCCAGGTTGCTCG
frag2_for: GGCTAGCGGAAAGAGGGGACAAATGTCAAAAATCC

frag3_rev: ATCAATGTAAATAATAAAAAAGCCGGATTAATAATCTGGCTTTTTATATTCT
frag3_for: TTAATAATCACACTGGCTCACCTTCGG

frag4_rev: CTCTTTCCGCTAGCCCAAAAAAACGGTATGGAG
frag4_for: ATTATTTACATTGATTATTTGCACGGCGT

frag5_rev: CGGCCGCTACTAGTATTATCATTTGTACAGCTCGTCCATGC
frag5_for: TTTTTTTGGGCTAGCGGAAAGAGGGGACAAATGATTAACCG

vector_rev: CTCTAGAAGCGGCCGCGA
vector_for: TACTAGTAGCGGCCGCTGC

Pre-PezT: GGATCCTGCAGCGGCCGCTACTAGTACTCGAGCGCTGCTGCCA
For-PezT: ATACCGAATTCGCGGCCGCTTCTAGATGGAAATCCAAGATTATACTG