June 04th 2013

The Aer-EnvZ piece after OEPCR is the correct size. After looking back at what Julie did (5/30 PCR and May 31 digestion) with OmpR, Brandon reran her 5/30 PCR. Brandon ran it at 58 C, 60 C, and 64 C. Julie originally used 60 C, and the two primers are supposed to have melting temperatures of 58 C and 67C. Tomorrow, assuming that OmpR works, Brandon will digest and ligate the pieces together.